Recombinant Human C-C Motif Chemokine 17 Protein (CCL17), Active

Name: Recombinant Human C-C Motif Chemokine 17 Protein (CCL17), Active
Brand: Beta LifeScience
SKU: BLC-05538P
Availability: InStock

Collections: All products, Buy cytokines, chemokines, and growth factors for research online, Chemokines and receptors – essential regulators of immune response, High-quality cytokines for advanced research, High-quality recombinant proteins

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Product Overview

Description	Recombinant Human C-C Motif Chemokine 17 Protein (CCL17), Active is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 97% as determined by SDS-PAGE and HPLC.
Endotoxin	Less than 1.0 EU/μg as determined by LAL method.
Activity	Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration range of 1.0-10 ng/ml.
Uniprotkb	Q92583
Target Symbol	CCL17
Synonyms	A-152E5.3; A152E53; ABCD 2; ABCD2; C-C motif chemokine 17; CC chemokine TARC; CCL17; CCL17_HUMAN; Chemokine CC Motif Ligand 17; MGC138271; MGC138273; SCYA17; Small Inducible Cytokine A17; Small Inducible Cytokine A17 Precursor; Small Inducible Cytokine Subfamily A (Cys Cys); Small Inducible Cytokine Subfamily A (Cys Cys) Member 17; Small-inducible cytokine A17; T Cell Directed CC Chemokine; Thymus and activation regulated chemokine ; Thymus and activation-regulated chemokine
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	Tag-Free
Complete Sequence	ARGTNVGREC CLEYFKGAIP LRKLKTWYQT SEDCSRDAIV FVTVQGRAIC SDPNNKRVKN AVKYLQSLER S
Expression Range	24-94aa
Protein Length	Full Length of Mature Protein
Mol. Weight	8.1 kDa
Research Area	Immunology
Form	Lyophilized powder
Buffer	Lyophilized from a 0.2 μm filtered 20mM PB, pH 7.4, 150mM NaCl
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
Subcellular Location	Secreted.
Protein Families	Intercrine beta (chemokine CC) family
Database References	HGNC: 10615 OMIM: 601520 KEGG: hsa:6361 STRING: 9606.ENSP00000219244 UniGene: PMID: 29848886 The expression of CCL17 was decreased in nasal polyp (NP) epithelium compared to the epithelium of normal ethmoid sinus, whereas the expression of CCL22 was not decreased. E-cadherin was differentially distributed between the epithelium of normal ethmoid sinus and NP epithelium. EGFR was also decreased in NPs. PMID: 29746583 TARC serum levels were elevated in 78% of sarcoidosis patients and correlated with disease severity. PMID: 29598931 Serum TARC level may be a potent marker reflecting better response to IL-17A inhibitors for psoriasis treatment. PMID: 29655215 This review presents key clinical studies of Multiple sclerosis together with experimental studies in animals that have demonstrated functional roles of CCR4, CCL17, and CCL22 in experimental autoimmune encephalomyelitis pathogenesis. [review] PMID: 29099057 Taken together, our data showed a likely positive feedback mechanism of enhanced IL-6 production in ectopic milieu. The high levels of IL-6 in the peritoneal cavity stimulate the expression of CCL17 in endometrial stromal cells by activating JNK signal pathway, and then, CCL17 further induces CCR4 expression on macrophages, which eventually leads to an increase in IL-6 production via NF-kappaB activation. PMID: 28240436 Data suggest that high levels of CCL17 and CCL22 in endometrium in women with endometriosis promote (1) recruitment of Tregs, (2) immunosuppression of Tregs, and (3) angiogenesis in endometrium. (Tregs = regulatory T cells) PMID: 28383711 Serum TARC levels correlate well with indicators of systemic inflammation and of disease severity among patients with a drug eruption except SJS/TEN. Serum TARC may be a prognostic biomarker of severity of inflammation in drug eruptions. PMID: 28648978 TARC production in HaCaT keratinocytes through the interaction between IL-22 and IL-22Ralpha facilitates T-cell migration in atopic dermatitis caused by house dust mites. PMID: 26914146 TARC is a candidate biomarker for PTSD only in males. PMID: 28170001 High CCL17 expression is associated with colon cancer cell migration. PMID: 28146427 GM-CSF can mediate inflammation and pain by regulating IRF4-induced CCL17 production PMID: 27525438 Based on the ADEPT study data set, we report that airway mucosal expression of CCL26 was a robust discriminator of type 2 inflammation from healthy nonatopic subjects. Furthermore, airway mucosal CCL26 expression was best identified by using a panel of clinical biomarkers, including Feno values, bEOS counts, and expression of 2 novel markers, sCCL17 and sCCL26 PMID: 28089872 Low CCL17 expression is a potential independent adverse prognostic biomarker for recurrence and survival of patients with clear cell renal cell carcinoma after nephrectomy. PMID: 28178948 IP-10 serum increase or TARC/IP-10 ratio decrease along with abnormal hepatic enzymatic alteration could signify early rejection of the transplanted liver. PMID: 28245475 elevated pre-treatment levels of sGal-1, sCD163, sCD30 and TARC can be found in patients with classic Hodgkin lymphoma. However, only plasma TARC accurately reflects disease activity and correlates with clinical treatment response. PMID: 27610595 levels elevated in rheumatoid arthritis synovial fluid, due to production by mononuclear cells, particularly CD1c classical dendritic cells PMID: 27250804 Serum TARC levels are well correlated with blood eosinophil counts in patients with generalized drug eruptions, indicating that Th2-type immune reactions underlie TARC production. Serum TARC measurements also have potent diagnostic value for DIHS, with high sensitivity and specificity. PMID: 27497618 Data suggest that Th1 (IFN-gamma-inducible protein-10 IP-10/CXCL10) and Th2 (thymus and activation regulated chemokine TARC/CCL17) and (macrophage derived chemokine MDC/CCL22) cooperatively play a role in the development of ankylosing spondylitis (AS). PMID: 26827189 CCL17 positively regulates the tumorigenesis of hepatocellular carcinoma.Higher levels of intratumoral CCL17 expression are associated with poorer survival of hepatocellular carcinoma patients. PMID: 26781124 CCL17 and CCL18 dermal expression is associated with leprosy polarity. PMID: 25412496 The SNP rs223895 in TARC/CCL17 gene is associated with the susceptibility to Kawasaki disease. PMID: 26182268 Serum CCL17 levels are associated with coronary artery disease and atherosclerosis severity independently of traditional cardiovascular risk factors. PMID: 25555269 TARC was correlated with disease status and its monitoring may be able to predict PET positivity after alloSCT, thus potentially allowing an early immune manipulation. PMID: 25240818 Serum levels of CCL17 were positively correlated with coronary artery disease. PMID: 25062565 increased expression in the thymus of myasthenia gravis patients PMID: 24397961 TARC and interleukin-31 may be biomarkers for adult atopic dermatitis PMID: 24984931 Serum TARC is a promising biomarker for remission and can be used for accurately monitoring proactive treatment for long-term control. [revew] PMID: 24628072 disease activity and response biomarker in alopecia areata PMID: 24102731 High CXCL10 levels were detected in acutely infected children regardless of virus pathogen, whereas increased CCL17 production was RSV-specific PMID: 25013801 Higher serum concentrations of CCL17 at baseline may be predictive of acute exacerbations in patients with chronic hypersensitivity pneumonitis PMID: 23705860 might be potential marker of non-eosinophilic oesophagitis gastrointestinal food allergies PMID: 24698291 Serum TARC may represent a suitable biomarker for monitoring of atopic dermatitis severity in daily practice. PMID: 25199679 Although TARC and I-TAC may not directly regulate pruritus in atopic dermatitis patients, these chemokines are very sensitive disease markers of AD. PMID: 24104601 we found that TARC is an easily measured biomarker that is associated with faster TTR, longer PFS, and possibly longer OS in a population of patients with HL who were treated with panobinostat. PMID: 23822537 The serum TARC level is a very sensitive biomarker for monitoring the severity and treatment response in AD PMID: 25268342 CCL17 is an antimicrobial protein with bacteriocidal activity against E. coli and S. aureus. PMID: 12949249 An association between TARC/CCL17 polymorphisms, susceptibility of Kawasaki disease, and intravenous immunoglobulin responses in Kawasaki disease patients. PMID: 23942559 Migration assays were performed to evaluate CCL17-induced colon cancer cell (HT-29) chemotaxis and RhoA protein levels were quantified. PMID: 24582560 Ginseng inhibited TARC expression via blockade of NF-kappaB activation in HaCaT cells. PMID: 23454147 CCL17-induced, CCR4-dependent release of CGRP by human airway epithelial cells represents a novel inflammatory pathway in patients with asthma and allergic disease. PMID: 23731651 These data reveal an elevated serum concentration of Th2-attracting chemokines CCL22 and CCL17 in opsoclonus-myoclonus syndrome. PMID: 23340773 Autistic children had significantly higher serum levels of TARC than healthy controls PMID: 23782855 Serum CD163 and TARC as disease response biomarkers in classical Hodgkin lymphoma. PMID: 23224400 Data suggest an important role for serum CCL17 level as biomarker of drug resistance/remission induction in Hodgkin lymphoma in response to various combined neoplastic agent protocols. PMID: 23225085 increased expression in macrophages from asthmatic patients PMID: 22981793 Data show that PD-1, PD-L1, PD-L2, CCL17, and CCL22 mRNA was identified in papillomas. PMID: 22322668 Serum concentrations of TARC and CTACK were significantly higher in AD (atopic dermatitis) children than in healthy controls, and correlated with severity of symptoms. PMID: 22017510 Baseline plasma thymus and activation-regulated chemokine levels correlate with classical Hodgkin's lymphoma tumor burden and serial levels correlate with response to treatment in patients with classical Hodgkin's lymphoma. PMID: 22058214 PI16-positive Treg showed enhanced in vitro migration towards the inflammatory chemokines CCL17 and CCL20, suggesting they can migrate to sites of inflammation PMID: 22533972

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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