Recombinant Human AURORA B (G160L) Protein
Beta LifeScience
SKU/CAT #: BL-0129SG
Recombinant Human AURORA B (G160L) Protein
Beta LifeScience
SKU/CAT #: BL-0129SG
Catalog No.: BL-0129SG
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NM_004217 |
| Synonym | AURKB, AIK2; AIM1; ARK2; AurB; IPL1; AIM-1; STK12 |
| Background | AURORA B is a member of the Aurora kinase family that associates with microtubules during chromosome movement and segregation. AURORA B localizes to the microtubules near kinetochores, specifically to the specialized microtubules called K-fibers (1). AURORA B inhibits the microtubule depolymerizing activity of mitotic centromere-associated kinesin (MCAK) by phosphorylating MCAK on Ser92 (2). This phosphorylation also regulates MCAK translocalization from kinetochores to the centromere. AURORA B has been identified as a target for the development of new anticancer agents since inhibition of AURORA B gives rise to the more pronounced antiproliferative phenotype. |
| Description | Recombinant full-length human AURORA B (G160L) was produced by baculovirus in Sf9 insect cells, fused with a GST tag at N-terminus. This protein is purified with our unique purification methods. |
| Source | Sf9 insect cells |
| AA Sequence | Full Length |
| Molecular Weight | ~68 kDa |
| Purity | For specific purity information on a given lot, see related COA. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Active |
| Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
| Stability | The recombinant protein is stable for up to 12 months at -70°C |
| Usage | For Research Use Only |
| Storage | Recombinant Human AURORA B (G160L) Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
