Recombinant Human ATP6V1F Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0290
Recombinant Human ATP6V1F Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0290
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | GST |
Host Species | Human |
Accession | NP_004222.2 |
Synonym | ATP6S14, VATF, Vma7 |
Background | ATP6V1F- encodes a component of vacuolar ATPase mediating acidification. The cDNA and the genomic sequences of- ATP6V1F- were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction and touchdown-polymerase chain reaction, respectively. Topology prediction showed that there is one protein kinase C phosphorylation site, two Casein kinase II phosphorylation sites, and one N-myristoylation site in the ATP6V1F protein.- Up-regulated expression of mammary tumor 8 kDa protein (MAT-8), complement component C1S (C1S), ferritin heavy chain (FTH1), peptidyl-prolyl cis-trans isomerase A (PPIA), RNA-binding protein regulatory subunit DJ-1 protein (DJ-1) and vacuolar ATP synthase subunit F (ATP6V1F) was determined in prostate carcinoma and confirmed by using quantitative real-time RT-PCR analyses. |
Description | A DNA sequence encoding the human ATP6V1F (NP_004222.2) (Met1-Arg119) was expressed with the GST tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | Met1-Arg119 |
Molecular Weight | The recombinant human ATP6V1F/GST chimera consists of 350 a.a. and has a predicted molecular mass of 40.2 KDa. It migrates as an approximately 40 KDa band in SDS-PAGE under reducing conditions. |
Purity | >90% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.4.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |