Recombinant Human ATP6V1F Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0290
Recombinant Human ATP6V1F Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0290
Catalog No.: BLPSN-0290
Product Overview
| Tag | GST |
| Host Species | Human |
| Accession | NP_004222.2 |
| Synonym | ATP6S14, VATF, Vma7 |
| Background | ATP6V1F- encodes a component of vacuolar ATPase mediating acidification. The cDNA and the genomic sequences of- ATP6V1F- were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction and touchdown-polymerase chain reaction, respectively. Topology prediction showed that there is one protein kinase C phosphorylation site, two Casein kinase II phosphorylation sites, and one N-myristoylation site in the ATP6V1F protein.- Up-regulated expression of mammary tumor 8 kDa protein (MAT-8), complement component C1S (C1S), ferritin heavy chain (FTH1), peptidyl-prolyl cis-trans isomerase A (PPIA), RNA-binding protein regulatory subunit DJ-1 protein (DJ-1) and vacuolar ATP synthase subunit F (ATP6V1F) was determined in prostate carcinoma and confirmed by using quantitative real-time RT-PCR analyses. |
| Description | A DNA sequence encoding the human ATP6V1F (NP_004222.2) (Met1-Arg119) was expressed with the GST tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Met1-Arg119 |
| Molecular Weight | The recombinant human ATP6V1F/GST chimera consists of 350 a.a. and has a predicted molecular mass of 40.2 KDa. It migrates as an approximately 40 KDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | Please contact us for more information. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
