Name: Recombinant Human ASF1B Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0278
Availability: InStock

Recombinant Human ASF1B Protein (His Tag)

Catalog No.: BLPSN-0278

Product Overview

Tag	His
Host Species	Human
Accession	Q9NVP2
Synonym	CIA-II
Background	The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which are required for the recruitment of RNF8/RNF168 histone ubiquitin ligases. Thus, ASF1a deficiency reduces histone ubiquitination at DSBs, decreasing the recruitment of 53BP1, and decreases NHEJ, rendering cells more sensitive to DSBs. This role of ASF1a in DSB repair cannot be provided by the closely related- ASF1b- and does not require its histone chaperone activity. Homozygous deletion of ASF1A is seen in 1%-15% of certain cancers, suggesting that loss of NHEJ may be selected in some malignancies and that the deletion can be used as a molecular biomarker for cancers susceptible to radiotherapy or to DSB-inducing chemotherapy. Anti-silencing function 1 (ASF1) is a histone H3-H4 chaperone involved in DNA replication and repair, and transcriptional regulation. Here, we identify ASF1B, the mammalian paralog to ASF1, as a proliferation-inducing histone chaperone in human beta-cells. Overexpression of ASF1B led to distinct transcriptional signatures consistent with increased cellular proliferation and reduced cellular death.
Description	A DNA sequence encoding the human ASF1B (Q9NVP2) (Met 1-Ile202) was expressed with a C-terminal His tag.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Met
AA Sequence	Met 1-Ile202
Molecular Weight	The secreted recombinant human ASF1B consists of 212 a.a. and predicts a molecular mass of 23.8 KDa. The apparent molecular mass of the protein is approximately 20 Kda in SDS-PAGE under reducing conditions due to glycosylation.
Purity	>90% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.