Recombinant Human ARF3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0251
Recombinant Human ARF3 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0251
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | P61204 |
Background | ARF3, also known as ADP-ribosylation factor 3, belongs to the RAS superfamily. Members of this family include ARF1, ARF2, ARF3, ARF4, ARF5 and ARF6. ARF3 gene is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF3 functions as an allosteric activator of the cholera toxin subunit, an ADP-ribosyltransferase. It is involved in protein trafficking and may modulate vesicle budding and uncoating within the Golgi apparatus. |
Description | A DNA sequence encoding the human ARF3 (P61204) (Met1-Lys181) was expressed with a His tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | His |
AA Sequence | Met1-Lys181 |
Molecular Weight | The recombinant human ARF3 consists of 196 a.a. and predicts a molecular mass of 22.4 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions. |
Purity | >85% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile 50mM Tris, 10% glycerol, pH 8.0.. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Target Details
Target Function | GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus. |
Subcellular Location | Golgi apparatus. Cytoplasm, perinuclear region. |
Protein Families | Small GTPase superfamily, Arf family |
Database References |
Gene Functions References
- Upregulation of ARF3 is associated with ovarian cancer. PMID: 28098897
- activated ARF3 is associated with Unc93B1 and TLR9, suggesting that ARF3 conducts TLR9 trafficking by forming the TLR9-Unc93B1-ARF3 complex. PMID: 26067373
- ARF1+ARF3 are required for integrity of recycling endosomes but are involved in distinct transport pathways: they are required for the transferrin recycling pathway from endosomes to the plasma membrane. PMID: 23783033
- These results suggest that Arf3 plays a unique function at the trans-Golgi network that likely involves recruitment by a specific receptor. PMID: 20357002
- expression and subcellular redistribution by heregulin PMID: 12135740