Recombinant Human APEG1 Protein (His Tag)

Name: Recombinant Human APEG1 Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0232
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	His
Host Species	Human
Accession	Q15772
Synonym	APEG-1, APEG1, BPEG, CNM5, SPEGalpha, SPEGbeta
Background	Striated muscle preferentially expressed protein kinase, also known as aortic preferentially expressed protein 1, APEG-1, SPEG and KIAA1297, is a protein which belongs to theprotein kinase superfamily and CAMK Ser/Thr protein kinase family. SPEG / APEG-1 contains twofibronectin type-III domains, nine Ig-like (immunoglobulin-like) domains, two protein kinase domains. Isoform1of SPEG is preferentially expressed in striated muscle. Non-kinase form such as isoform3of SPEG is predominantly expressed in the aorta. Isoform3of SPEG appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dedifferentiated ASMC. Isoform3of SPEG may have a role in regulating the growth and differentiation of arterial smooth muscle cells. Isoform3of SPEG is quickly down-regulated in response to vascular injury, when ASMC cells change from a quiescent to a proliferative phenotype.
Description	A DNA sequence encoding the human SPEG isoform 3 (Q15772-4) (Met 1-Glu 113) was fused with a His tag at the C-terminus.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Met 1-Glu 113
Molecular Weight	The recombinant human SPEG consisting of 123 a.a. and has a calculated molecular mass of 14 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions.
Purity	>85% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile PBS, pH 7.4.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Isoform 3 may have a role in regulating the growth and differentiation of arterial smooth muscle cells.
Subcellular Location	[Isoform 3]: Nucleus.
Protein Families	Protein kinase superfamily, CAMK Ser/Thr protein kinase family
Database References	HGNC: 16901 OMIM: 615950 KEGG: hsa:10290 STRING: 9606.ENSP00000311684 UniGene: Hs.21639
Associated Diseases	Myopathy, centronuclear, 5 (CNM5)
Tissue Specificity	Isoform 1 is preferentially expressed in striated muscle. Non-kinase form such as isoform 3 is predominantly expressed in the aorta. Isoform 3 appears to be expressed only in highly differentiated ASMC in normal vessel walls and down-regulated in dediffer

Gene Functions References

SPEG is present in cardiac muscle, where it plays a critical role; therefore, individuals with SPEG mutations additionally present with dilated cardiomyopathy. PMID: 25087613
Genomic rearrangement on APEG1 in arteriosclerosis was studied. PMID: 15784173
the RGD motif might play a role not only in the adhesion of Aortic Preferentially Expressed Protein-1 and extracellular proteins but also in intracellular protein-protein interactions PMID: 16354304

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.