Recombinant Human APE1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0231
Recombinant Human APE1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0231
Collections: Other recombinant proteins, Recombinant proteins
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | P27695 |
| Synonym | APE, APE1, APEN, APEX, APX, HAP1, REF1 |
| Background | The enzyme is known to be a redox factor (Ref-1) stimulating DNA binding activity of AP-1 binding proteins such as Fos and Jun as well as a multifunctional DNA repair enzyme having 5' AP endonuclease, DNA 3' repair diesterase, 3'-5' exonuclease and DNA 3'-phosphatase activities.Although Apex mRNA was expressed ubiquitously, the levels varied significantly, suggesting organ- or tissue-specific expression of the Apex gene. The highest level was observed in the testis, relatively high levels in the thymus, spleen, kidney and brain, and the lowest level in the liver in rats. However, the present results suggested that APEX/Ref-1 gene product can interact with AP-1 binding proteins in brain, especially in the hippocampal formation, to regulate some brain functions by redox-activation. |
| Description | A DNA sequence encoding the human APEX1 (P27695) (Pro2-Leu 318) was expressed, with a His tag at the N-terminus. |
| Source | E.coli |
| Predicted N Terminal | Met |
| AA Sequence | Pro2-Leu 318 |
| Molecular Weight | The recombinant human APEX1 consisting of 328 a.a. and migrates as an approximately 37 kDa band in SDS-PAGE under reducing conditions as predicted. |
| Purity | >92% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.5. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
