Recombinant Human ANGPTL8 Protein (N-Fc)

Name: Recombinant Human ANGPTL8 Protein (N-Fc)
Brand: Beta LifeScience
SKU: BL-1834NP
Availability: InStock

BL-1834NP: Greater than 70% as determined by reducing SDS-PAGE. (QC verified)

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Angiopoietin-like Protein 8 is produced by our Mammalian expression system and the target gene encoding Ala22-Ala198 is expressed with a human IgG1 Fc tag at the N-terminus.
Accession	Q6UXH0
Synonym	Betatrophin;Angiopoietin-like protein 8;Lipasin;Angptl8
Gene Background	The protein specifically promotes pancreatic beta cell proliferation and beta cell mass expansion, thereby improving glucose tolerance. It promotes pancreatic beta cell proliferation without insulin resistance. Also it acts as a blood lipid regulator by regulating serum triglyceride levels and possibly by promoting ANGPTL3 cleavage. It interacts with ANGPTL3. It predominantly expressed in liver and also expressed in adipose tissues. The ability of the protein to induce pancreatic beta cell proliferation is promising in diabetes therapy. Betatrophin treatment could supply or replace insulin injections by increasing the number of insulin-producing cells in diabetes.
Molecular Mass	46 KDa
Apmol Mass	54 KDa, reducing conditions
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Endotoxin	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Purity	Greater than 70% as determined by reducing SDS-PAGE. (QC verified)
Biological Activity	Not tested
Reconstitution	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Usage	For Research Use Only

Target Details

Target Function	Hormone that acts as a blood lipid regulator by regulating serum triglyceride levels. May be involved in the metabolic transition between fasting and refeeding: required to direct fatty acids to adipose tissue for storage in the fed state.
Subcellular Location	Secreted.
Protein Families	ANGPTL8 family
Database References	HGNC: 24933 OMIM: 125853 KEGG: hsa:55908 STRING: 9606.ENSP00000252453 UniGene: PMID: 28600576 Inhibition of miR-143-3p amplified ANGPTL8 response to treatments (glucose, insulin, Lipopolysaccharides), suggesting that the miRNA acts to suppress ANGPTL8 expression under metabolically distorted conditions. PMID: 30261196 Study suggests that ANGPTL8 levels in early pregnancy are significantly and independently associated with risk of GDM at 24-28 weeks of gestation. PMID: 29167926 Circulating full-length ANGPTL8 concentrations in patients with dyslipidemia were significantly elevated as compared to controls. PMID: 30021605 ApoCIII may mediate the effects of ANGPTL8 on triglyceride metabolism. PMID: 30021607 Study shows that angiopoietin-like protein 8 (ANGPTL-8) was positively correlated with hepatocellular lipid content independent of obesity and insulin resistance, indicating that ANGPTL-8 might be a new and important important predictor of the severity of non-alcoholic fatty liver disease. PMID: 29266821 We conclude that serum betatrophin concentrations were increased in pregnancies affected by hyperemesis gravidarium. PMID: 29754072 Data suggest the angiopoietin-like 8 (ANGPTL8)/p62-IKKgamma axis as a negative feedback loop that regulates NF-kappaB activation, and extends the role of selective autophagy in fine-tuned inflammatory responses. PMID: 29255244 ANGPTL8 levels are increased in both plasma and adipose tissues of subjects with hypertension. PMID: 29490644 Serum ANGPTL8 concentrations were significantly increased in impaired glucose regulation and type 2 diabetes. PMID: 29082263 the level of circulating betatrophin is positively associated with insulin resistance. PMID: 28931172 we identified ANGPTL8 as the target gene at this HDL-C GWAS locus, determined regulatory drivers of tissue specificity, and combined fine-mapping approaches and regulatory overlap with experimental assays to identify variants that may contribute to the HDL-C GWAS signal at ANGPTL8. PMID: 28754724 serum levels not associated with weight status, glucose tolerance, insulin resistance, or lipid metabolism PMID: 27402552 Elevated betatrophin levels in polycystic ovary syndrome women, in the absence of obesity and glucose intolerance, may reflect a compensatory mechanism in order to counteract metabolic syndrome-related risk factors. PMID: 28319674 betatrophin levels appeared to be related to the pathogenesis of the diabetic stages rather than prediabetic stages. PMID: 28351091 Angiopoietin-like protein 8/betatrophin is related to liver steatosis, while visceral adipose tissue represents an additional site of expression in humans. PMID: 28351093 ANGPTL8 reverses established Adriamycin induced cardiomyopathy by stimulating adult cardiac progenitor cells. PMID: 27823982 Study provided an update to understand its physiological function from literature curated findings through an integrated view of ANGPTL8's regulation and its associated pathways. PMID: 28684091 betatrophin is increased in pancreatic cancer-associated diabetes PMID: 27276680 These results confirm a role for ANGPTL8 in lipoprotein metabolism and provide novel support for functional consequences of the rs2278426 variant. PMID: 27117576 ANGPTL8 has a functional LPL inhibitory motif, but only inhibits LPL and increases plasma TG levels in mice in the presence of ANGPTL3 PMID: 28413163 The inflammation-induced miR-221-3p regulates ANGPTL8 expression in adipocytes. This miRNA impact may become especially prominent under pathologic conditions such as morbid obesity, putatively contributing to the impaired adipose tissue lipid metabolism in metabolic disease. PMID: 28938482 Circulating ANGPTL8 increases after bariatric surgery and predicts type 2 diabetes remission in morbidly obese patients. PMID: 28347650 Data suggest that plasma betatrophin levels are significantly increased in lean (normal-weight) women with PCOS (polycystic ovary syndrome) compared to lean women without PCOS; plasma betatrophin levels are not significantly altered in overweight/obese women with PCOS compared to overweight/obese women without PCOS. This study was conducted in China. PMID: 27960599 Especially the association to eGFR highlights the importance for future studies to address renal function as possible influence on betatrophin regulation and consider eGFR as potential confounder when analyzing the role of betatrophin in humans PMID: 28257453 Serum betatrophin is an independent risk factor for nonalcoholic fatty liver and potential non-invasive marker for its progression. PMID: 28125672 Cord blood betatrophin may function as a potential biomarker of maternal intrauterine hyperglycemia and fetal insulin resistance, which may presage for long-term metabolic impact of gestational diabetes on offspring PMID: 27196053 The high levels of ANGPTL8 found in fetal life together with its relationship with newborn adiposity and brown adipose tissue point to ANGPTL8 as a potential new player in the modulation of the thermogenic machinery during the fetal-neonatal transition. PMID: 27469268 Betatrophin levels were increased in women with PCOS and were associated with insulin resistance, c-reactive protein, and free-testosterone in these patients. PMID: 26832343 Obesity is associated with increased betatrophin suppression after an oral glucose load; this response appears to be driven by hyperglycemia. Data suggest that the impaired betatrophin response in obese subjects is restored after weight loss and is comparable with the betatrophin response in lean individuals. PMID: 27459526 Serum betatrophin levels are increased and associated with insulin resistance in patients with polycystic ovary syndrome. PMID: 28222635 ANGPTL8/betatrophin might play an important role in glucose metabolism in the context of insulin resistance. PMID: 26387753 This study supports that serum betatrophin plays an important role in MetS, involving the regulations of glucose and lipid metabolism and inflammation. PMID: 27238790 our data shows that ANGPTL3, 4 and 8 are increased in obesity and type 2 diabetes (T2D). ANGPTL8 associates with ANGPTL3 in the non-diabetic subjects while it associated more with ANGPTL4 in the obese and T2D subjects. PMID: 27733177 circulating betatrophin is increased in mice and humans with NAFLD and its expression was induced by endoplasmic reticulum stress in hepatocytes PMID: 27045862 High betatrophin expression is associated with diabetic metabolic syndrome. PMID: 27578619 Elevated betatrophin levels were associated with cardiometabolic risk factors a young population, but the association was largely dependent on vitamin D status. PMID: 27716289 betatrophin may be associated with thyroid insufficiency but not thyroid autoimmunity. PMID: 27213151 Betatrophin levels in patients with polycystic ovary syndrome (PCOS) are lower than those without PCOS and inversely related to insulin resistance. PMID: 27188865 data shows for the first time that heterozygote form of ANGPTL8 Rs.2278426 variant was associated with higher FBG level in Arabs highlighting the importance of these variants in controlling the function of betatrophin. PMID: 26864934 Serum betatrophin concentrations are increased in type 2 diabetes patients under antidiabetic treatment and positively associated with diabetic retinopathy. PMID: 26863068 Compared with type 2 diabetes without cardiovascular disease (CVD), patients with CVD had remarkably higher levels of angiopoietin-like protein 8 (ANGPTL8). PMID: 27596060 Circulating betatrophin concentration is negatively correlated with insulin resistance in obese children and adolescents. PMID: 27103367 Meta-analysis results from case control studies on betatrophin and type 2 diabetes (T2DM) revealed increased circulating levels of betatrophin in patients with T2DM. PMID: 27242389 ANGPTL8 levels were associated with baseline and future changes in triglyceride levels in Korean children. PMID: 26739706 Betatrophin is significantly increased in T2DM patients with different stages of albuminuria. Betatrophin may be a novel endocrine regulator involved in DN development. PMID: 26739836 ANGPTL8 is increased in subjects with MetS and it was significantly associated with HsCRP levels in different subgroups. PMID: 26850725 Disruption of ANGPTL8 function in humans does not seem to have a large effect on measures of glucose tolerance. PMID: 26822414 Based on the findings of our meta-analysis, circulating betatrophin level of T2DM patients is higher than that of nondiabetic adults in the nonobese population, but not in the obese population. PMID: 26697500 In patients with diabetes mellitus type 2, betatrophin levels did not correlate with beta-cell function-related variables or insulin resistance-related variables PMID: 26649318

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human ANGPTL8 Protein (N-Fc)

Recombinant Human ANGPTL8 Protein (N-Fc)

Product Overview

Target Details

FAQs