Recombinant Human ANGPTL2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0209
Recombinant Human ANGPTL2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0209
Catalog No.: BLPSN-0209
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_036230.1 |
| Synonym | ARP2, HARP |
| Background | The angiopoietin-like protein (ANGPTL) family is homologous to angiopoietins but does not bind to the Tie2 receptor. The function of ANGPTLs has been elucidated largely in the context of angiogenesis and lipid metabolism. Morinaga et al. demonstrated that genetic depletion of Angptl2 confers amelioration of the mouse kidney fibrosis induced by a unilateral ureteral obstruction, implicating that ANGPTL2, predominantly in the renal tubular compartments, activates the transforming growth factor-beta signaling and vice versa through miR-221. Angiopoietin-like protein- 2- (ANGPTL2) maintains tissue homeostasis by inducing inflammation and angiogenesis. It is produced in infiltrating immune cells or resident cells, such as adipocytes, vascular endothelial cells, and tumor cells.- The classic sequential cascade of P. gingivalis LPS -†’ inflammatory cytokine induction is well established. However, in the current study, we reveal a novel cascade comprising sequential P. gingivalis LPS -†’ ANGPTL2 -†’ integrin alpha5beta1 -†’ inflammatory cytokine induction, which might be responsible for inducing potent periodontal disorganization activity in gingival epithelial cells. Via this pathway, ANGPTL2 functions in the pathogenesis of periodontitis and contributes to prolonging chronic inflammation in patients with systemic disease. That MAC-3-positive immune cells, including infiltrating bone marrow-derived macrophages and activated microglia, express abundant angiopoietin-like protein (ANGPTL) 2 in ischemic mouse brain in a transient middle cerebral artery occlusion (MCAO) model. Both neurological deficits and infarct volume decreased in transient MCAO model mice established in Angptl2 knockout (KO) relative to wild-type mice. Acute brain inflammation after ischemia-reperfusion, as estimated by expression levels of pro-inflammatory cytokines such as interleukin (IL)-1beta and tumor necrosis factor alpha (TNF)-alpha, was significantly suppressed in Angptl2 KO compared to control mice. |
| Description | A DNA sequence encoding the human ANGPTL2 (NP_036230.1) (Asp245-His493) was expressed with a His tag at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | His |
| AA Sequence | Asp245-His493 |
| Molecular Weight | The recombinant human ANGPTL2 consists of 268 a.a. and predicts a molecular mass of 31.4 kDa. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
