Recombinant Human Angiopoietin-2 / AGPT2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0197
Recombinant Human Angiopoietin-2 / AGPT2 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0197
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | His |
Host Species | Human |
Accession | NP_001138.1 |
Synonym | AGPT2, ANG2, Angiopoietin-2 |
Background | Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression. |
Description | A DNA sequence encoding the human Angiopoietin 2 (NP_001138.1) (Met 1-Phe 496) was expressed, fused with a His tag at the C-terminus. |
Source | HEK293 |
Predicted N Terminal | Tyr 19 |
AA Sequence | Met 1-Phe 496 |
Molecular Weight | The recombinant human Ang2 consists of 489 a.a. and predicts a molecular mass of 56.3 kDa. As a result of glycosylation, the rhAng2 migrates as approximately 60-70 kDa band in SDS-PAGE under reducing conditions. |
Purity | >92% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | 1. Measured by its ability to bind recombinant human Tie2-His in a functional ELISA.2. Measured by its ability to bind recombinant human Tie2-Fch in a functional ELISA. |
Formulation | Lyophilized from sterile PBS, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |