Human ANGPT1 (Angiopoietin-1) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-08475P
Beta Lifescience recombinant protein notice with SDS-PAGE availability in next QC run
Human ANGPT1 (Angiopoietin-1) - Recombinant Protein
Beta LifeScience
SKU/CAT #: BLT-08475P
Regular price
$59500
$595.00
Sale price$44500
$445.00Save $150
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Quantity Pricing
| Pack Size | Price (USD) |
|---|---|
| 500 µg | $1,030 (Fall Promotion) |
| 1 mg | $1,870 (Fall Promotion) |
For direct online orders, quantity pricing will be displayed in cart when you add 5x100ug or 10x100ug
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Product Overview
| Product Name | Recombinant Human Angiopoietin 1 (ANGPT1) Protein |
| Product Overview | This recombinant human Angiopoietin 1 (ANGPT1) protein includes amino acids 21-228 of the target gene is expressed in E.coli.The protein is supplied in lyophilized form and formulated in PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitolprior to lyophilization. |
| Target Uniprot Id | Q15389 |
| Recommended Name | Angiopoietin-1 |
| Gene Name | ANGPT1 |
| Synonyms | ANG1; AGP1; AGPT; ANG-I |
| Species | Human |
| Predicted Molecular Mass | 26kDa |
| Expression System | E.coli |
| Expression Range | 21-228 |
| Tag | N-6His |
| Purity | >90% |
| Formulation | Lyophilized |
| Buffer | PBS pH 7.4, 0.01% SKL, 5% Trehalose, 1% Mannitol |
| Storage Condition | 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C. |
| Reconstitution Instruction | Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%. |
| Applications | Positive Control; Immunogen; SDS-PAGE; WB |
| Research Area | Cardiovascular |
| Target Function | Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. |
| Subcellular Location | Secreted. |
