Recombinant Human AMPK alpha 1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-0182
Recombinant Human AMPK alpha 1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-0182
Collections: Other recombinant proteins, Recombinant proteins
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Product Overview
Tag | N/A |
Host Species | Human |
Accession | P54619 |
Synonym | AMPK, AMPKa1 |
Background | The PRKAA1 gene encodes the catalytic alpha-subunit of 5' AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor that maintains energy homeostasis within the cell and is activated when the AMP/ATP ratio increases. Moreover, AMPK has been shown to be involved in inhibiting tumour growth and metastasis, and has also been implicated in the pathology of neurodegenerative and cardiac disorders. All 5 tested tag SNPs of the PRKAA1 gene (rs1336177, rs154268, rs385486, rs688293, and rs174991) were significantly associated with gastric cancer.An inhibitor of PRKAA1 promoted the secretion of IL6, whereas an agonist of PRKAA1 suppressed the production of IL6. |
Description | A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331), constructed the plasmid 1; A DNA sequence encoding the human PRKAB2 (O43741) (Met 1-Ile 272), constructed the plasmid 2; A DNA sequence encoding the human PRKAA1 (Q13131-1) (Met 1-Gln 559) was fused with the N-terminal His-tagged GST tag at the N-terminus, constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified. |
Source | Baculovirus-Insect Cells |
Predicted N Terminal | Met & Met & His |
AA Sequence | Met 1-Pro 331 |
Molecular Weight | The recombinant heterotrimer of human AMPK (PRKAG1 / PRKAB2 / PRKAA1) has a calculated molecular mass of 160 (38+30+92) KDa. The apparent molecular mass is approximately 35, 37 & 95 KDa respectively in SDS-PAGE under reducing conditions. |
Purity | >94% as determined by SDS-PAGE |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Bioactivity | The specific activity was determined to be 6 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate. |
Formulation | Supplied as sterile 50mM Tris, 200mM NaCl, 2mM GSH, 1mM EDTA, 1mM DTT, 10% gly, pH 7.4. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |