Recombinant Human Aminopeptidase A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0177
Recombinant Human Aminopeptidase A Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0177
Catalog No.: BLPSN-0177
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | NP_001968.3 |
| Synonym | APA, CD249, ENPEP, gp160 |
| Background | ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure. |
| Description | A DNA sequence encoding the human ENPEP (NP_001968.3) (Arg41-Gly957) was expressed with a His tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | His |
| AA Sequence | Arg41-Gly957 |
| Molecular Weight | The recombinant human ENPEP consists of 933 a.a. and has a calculated molecular mass of 107.2 kDa. The recombinant protein migrates as an approximately 115.5 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >90% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin (Glu-AMC). The specific activity is >2000 pmoles/min/ug. |
| Formulation | Lyophilized from sterile 20 mM, Tris 500 mM NaCl, 10 % glycerol, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
