Recombinant Human Amine Oxidase [Flavin-Containing] B (MAOB) Protein (His)

Name: Recombinant Human Amine Oxidase [Flavin-Containing] B (MAOB) Protein (His)
Brand: Beta LifeScience
SKU: BLC-04672P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of this product could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) MAOB.

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Product Overview

Description	Recombinant Human Amine Oxidase [Flavin-Containing] B (MAOB) Protein (His) is produced by our E.coli expression system. This is a cytoplasmic protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P27338
Target Symbol	MAOB
Synonyms	Adrenalin oxidase; Amine oxidase (flavin containing); Amine oxidase [flavin-containing] B; AOFB_HUMAN; MAO, brain; MAO, platelet; MAO-B; MAOB; MGC26382; Monoamine oxidase B; Monoamine oxidase type B; OTTHUMP00000023166; Tyramine oxidase
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	SNKCDVVVVGGGISGMAAAKLLHDSGLNVVVLEARDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEVERLIHHVKGKSYPFRGPFPPVWNPITYLDHNNFWRTMDDMGREIPSDAPWKAPLAEEWDNMTMKELLDKLCWTESAKQLATLFVNLCVTAETHEVSALWFLWYVKQCGGTTRIISTTNGGQERKFVGGSGQVSERIMDLLGDRVKLERPVIYIDQTRENVLVETLNHEMYEAKYVISAIPPTLGMKIHFNPPLPMMRNQMITRVPLGSVIKCIVYYKEPFWRKKDYCGTMIIDGEEAPVAYTLDDTKPEGNYAAIMGFILAHKARKLARLTKEERLKKLCELYAKVLGSLEALEPVHYEEKNWCEEQYSGGCYTTYFPPGILTQYGRVLRQPVDRIYFAGTETATHWSGYMEGAVEAGERAAREILHAMGKIPEDEIWQSEPESVDVPAQPITTTFLERHLPSV
Expression Range	2-489aa
Protein Length	Cytoplasmic Domain
Mol. Weight	59.3kDa
Research Area	Neuroscience
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOB preferentially degrades benzylamine and phenylethylamine.
Subcellular Location	Mitochondrion outer membrane; Single-pass type IV membrane protein; Cytoplasmic side.
Protein Families	Flavin monoamine oxidase family
Database References	HGNC: 6834 OMIM: 309860 KEGG: hsa:4129 STRING: 9606.ENSP00000367309 UniGene: PMID: 29321361 This study shows that MAO-B levels are increased not only in astrocytes but also in pyramidal neurons in Alzheimer disease brain and also suggests that MAO-B regulates Abeta production in neurons via gamma-secretase PMID: 28764767 The findings may suggest that MAO-B is an important sensor in iron-stressed neuronal cells. PMID: 28685208 brain protein levels of MAOB are normal or elevated in the three parkinsonian conditions-with MAOB increase generally associated with elevations in levels of astrocyte markers. Brain MAOA concentrations were, somewhat surprisingly, not decreased in Parkinson's disease, Progressive Supranuclear palsy or multiple system atrophy with the exception of the atrophic putamen in MSA, despite loss of dopamine neurons. PMID: 29050386 MAOB gene variants are contributing to the etiology of ADHD in the Indo-Caucasoid population PMID: 27341797 This study suggests that MAOB increases ASD risk in males, possibly through its sex-specific regulatory effect on 5-HT metabolism and behavior. PMID: 27381555 These findings suggest an association of platelet MAO-B activity, but a lack of association of MAOB rs1799836 and MAOA-uVNTR, with selected psychotic symptoms in ethnically homogenous veterans with PTSD. PMID: 27112218 No significant association was found between severe agitation and MAOA uVNTR or MAOB rs1799836 polymorphism, revealing that these individual polymorphisms in MAO genes are not related to severe agitation in subjects with schizophrenia and conduct disorder. Higher platelet MAO-B activity was found in subjects with severe agitation vs. non-agitated subjects. Platelet MAO-B activity was not associated with MAOB rs1799836 PMID: 26851573 we performed a meta-analysis in the Chinese population to provide comprehensive data on the association between the MAOB polymorphism and Parkinson's disease. Overall, no significant association was found between the MAOB A644G polymorphism and Parkinson's disease risk in the Chinese population. PMID: 27421021 inhibition of MAO-B using selegiline reversed the cigarette smoke-induced oxidative stress and inflammation in bronchial epithelial cells. PMID: 28108387 Monoamine oxidase B levels are highly expressed in gliomas. PMID: 26689994 The results of this meta-analysis suggest that people in the Asian population with the MAOB intron 13 A allele have an increased risk of Parkinson disease, especially when combined with the COMT LL genotype. PMID: 26000819 Report isolation of MOA-B inhibitors from Vernonia cinerea. PMID: 25857233 MAOB A644G polymorphism was associated with Parkinson Disease risk and a subgroup analyses by ethnicity and gender also found significant relationships between this polymorphism and PD risk. PMID: 24658549 Data (including data from molecular dynamics simulation studies) suggest that the protonation state of the active site residue Lys296 in monoamine oxidase B does not have influence on the hydride transfer reaction in the metabolism of dopamine. PMID: 25220264 Polymorphisms of COMT and MAO-B genes has a unique characteristics among Chinese patients with Parkinson's disease. They may be related with differences in drug response in such patients. PMID: 25636089 There is significantly decreased expression of MAO-A and MAO-B in cancerous tissues when compared with adjacent noncancerous tissues. PMID: 25389533 The results of this study suggested that polymorphisms in MAOB may increase the risk of wearing-off and dyskinesias. PMID: 25034874 study revealed increased platelet MAO-B activity in patients with alcohol dependence; suggests increased platelet MAO-B activity might be used as independent peripheral indicator of alcohol dependence PMID: 25035107 We found significant negative correlations regarding the expression of the genes COMT, MAOB, DRD4, DRD5 and FOS, indicating that increased schizotypy coincides with higher levels of dopaminergic dysregulation on the mRNA-level. PMID: 24630741 Our results confirmed that SNPs in DRD4 and COMT, but not DBH or MAOB are associated with autism and ADHD in small, ethnically homogeneous groups of non-related male Caucasians. PMID: 24210742 genetic variants of COMT, MAOB and DRD2 loci modulate susceptibility to PD in South Indian subjects PMID: 24772965 Data indicate that in vitro MAO-B inhibitory activity by synthesized (coumarin-3-yl)carbamates is comparable with that of the selegiline. PMID: 23474901 The present data suggest that pharmacokinetic or pharmacodynamic factors other than the investigated genetic variants of the MAOB and COMT enzymes seem to determine the response to levodopa in the Iranian PD patients. PMID: 23093014 data may suggest that the MAOB and COMT genetic polymorphisms do not play any role in the pathogenesis of Parkinson's disease in Iranians PMID: 23319194 The rs1799836 SNP did not appear to correlated with attentional bias for facial expressions. PMID: 23054588 results provide additional evidence that GABRB3 and MAOB/NDP gene regions might constitute risk factors for hallucinations and delusions in schizophrenia. PMID: 22414661 G/A dimorphism in intron 13 sequence creates splicing enhancer thus stimulating intron 13 removal efficiency PMID: 22974659 [review] While MAO-B primarily serves in the catabolism of 2-phenylethylamine and contributes to the degradation of other trace amines and dopamine, MAO-A has high affinity for serotonin and norepinephrine. PMID: 21971001 Mao-B platelet protein level may serve as a biomarker for age-related dementia, especially AD. PMID: 22270014 [review] A better understanding of the transcriptional regulation of MAO B may help explain the differential tissue-specific expression of the two isoenzymes and provide insights into the molecular basis of the disorders associated with MAO dysfunction. PMID: 21359973 Results suggest MAOB is a susceptibility gene for schizophrenia in Han Chinese. PMID: 21978760 results demonstrate that the bipartite cavity structure in MAO B plays an important role in substrate and inhibitor recognition to distinguish its specificities from those of MAO A PMID: 21978362 Nitrogen kinetic isotope effects for the oxidation of benzylamine and (1,1-(2)H(2))benzylamine by recombinant human monoamine oxidase B show that cleavage of the CH bond is not concerted with rehybridization of the nitrogen atom. PMID: 21786798 mutation in gene encoding the monoamine degradation enzyme monoamine oxidase B was found in SCZ. PMID: 20479760 COMT rs4680 and MAOB rs1799836 polymorphisms may increase susceptibility to Parkinson's disease risk among Japanese PMID: 21781348 In early stage Alzheimer disease, discrete MAO-B reduction reflects the capacity of the population of MAO-B-positive progenitor cells to adapt to the neurodegenerative process. PMID: 20648649 a monoamine-oxidase epigenetic regulation is involved in effects of smoking PMID: 19956754 Children with different subtypes of ADHD had significantly lower platelet MAO-B activity than control children. PMID: 20022119 Data show that lower p-MAO-B associated with increased inattention scores (Conners' teacher-rating scale) and lower l-MR associated with increased score for oppositional-defiant disorder. PMID: 19863190 first evidence that MAOB polymorphisms influence levels of negative emotionality in healthy human volunteers, may lead to a better understanding of personality traits and susceptibility to developing psychiatric disorders such as major depression PMID: 19657584 Inflammation of the dental pulp was accompanied by a significant decrease in MAO labeling, MAO B (but not MAO A) activity and the increase in SSAO activity. PMID: 19789505 X ray structure to 3 A resolution PMID: 11753429 Association of monoamine oxidase A gene polymorphism with Alzheimer's disease and Lewy body variant. PMID: 12098640 a strong gender difference exists with respect to the modifying effect of MAO-B genotype on the smoking association with parkinson disease PMID: 12428723 MAO-B polymorphisms are associated with smoking behaviour PMID: 12563176 There was no interaction of smoking and the G allele and risk of Parkinson disease. PMID: 12815741 molecular models and binding sites of inhibitors PMID: 12825788 MAO B gene expression is selectively induced by a decreased Sp3/Sp1 ratio and reduced DNA methylation PMID: 12855685 The 1.7-A structure of the reversible isatin-MAO-B complex has been determined; it forms a basis for the interpretation of the enzyme's structure when bound to either reversible or irreversible inhibitors. PMID: 12913124

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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Recombinant Human Amine Oxidase [Flavin-Containing] B (MAOB) Protein (His)

Recombinant Human Amine Oxidase [Flavin-Containing] B (MAOB) Protein (His)

Product Overview

Target Details

FAQs