Recombinant Human Amelotin Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0171
Recombinant Human Amelotin Protein (Fc Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0171
Catalog No.: BLPSN-0171
Product Overview
| Tag | Fc |
| Host Species | Human |
| Accession | NP_997722.1 |
| Synonym | UNQ689 |
| Background | Amelotin (AMTN) is a tooth enamel protein which is expressed in maturation-stage ameloblasts and also in the internal basal lamina of junctional epithelium, a unique epithelial structure attached to the tooth surface which protects against the constant microbiological challenge to the periodontium. TNF-alpha stimulates AMTN gene transcription in human gingival epithelial cells via C/EBP1, C/EBP2, and YY1 elements in the human AMTN gene promoter.AMTN mRNA levels increased at 6 h and reached maximum at 24 h in GE1 cells. Luciferase activities of the mouse AMTN gene promoter constructs were induced by TGFbeta1. AMTN mRNA levels were induced at the initiation of apoptosis by TGFbeta1, which mediated through the Smad3 bindings to SBEs in the mouse AMTN gene promoter. Amelotin (Amtn) is a recently identified enamel protein secreted by ameloblasts at late stage of enamel development. Runt-related transcription factor 2 (Runx2) in combination with the coactivator core-binding factor beta (Cbfbeta) regulates the early stages of tooth development. |
| Description | A DNA sequence encoding the human AMTN (NP_997722.1) (Met1-Gln209) was expressed with the Fc region of mouse IgG1 at the C-terminus. |
| Source | HEK293 |
| Predicted N Terminal | Leu 17 |
| AA Sequence | Met1-Gln209 |
| Molecular Weight | The recombinant human AMTN/mFc comprises 427 a.a. and has a predicted molecular mass of 46.2 kDa. The apparent molecular mass of the monomer is approximately 54 kDa in SDS-PAGE under reducing conditions. |
| Purity | >85% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
