Recombinant Human All-trans-retinol dehydrogenase [NAD (ADH7) Protein (His)

Name: Recombinant Human All-trans-retinol dehydrogenase [NAD (ADH7) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09534P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Human All-trans-retinol dehydrogenase [NAD (ADH7) Protein (His) is produced by our Yeast expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P40394
Target Symbol	ADH7
Synonyms	ADH7; All-trans-retinol dehydrogenase [NAD(+)] ADH7; EC 1.1.1.105; Alcohol dehydrogenase class 4 mu/sigma chain; EC 1.1.1.1; Alcohol dehydrogenase class IV mu/sigma chain; Gastric alcohol dehydrogenase; Omega-hydroxydecanoate dehydrogenase ADH7; EC 1.1.1.66; Retinol dehydrogenase
Species	Homo sapiens (Human)
Expression System	Yeast
Tag	N-6His
Target Protein Sequence	MFAEIQIQDKDRMGTAGKVIKCKAAVLWEQKQPFSIEEIEVAPPKTKEVRIKILATGICRTDDHVIKGTMVSKFPVIVGHEATGIVESIGEGVTTVKPGDKVIPLFLPQCRECNACRNPDGNLCIRSDITGRGVLADGTTRFTCKGKPVHHFMNTSTFTEYTVVDESSVAKIDDAAPPEKVCLIGCGFSTGYGAAVKTGKVKPGSTCVVFGLGGVGLSVIMGCKSAGASRIIGIDLNKDKFEKAMAVGATECISPKDSTKPISEVLSEMTGNNVGYTFEVIGHLETMIDALASCHMNYGTSVVVGVPPSAKMLTYDPMLLFTGRTWKGCVFGGLKSRDDVPKLVTEFLAKKFDLDQLITHVLPFKKISEGFELLNSGQSIRTVLTF
Expression Range	1-386aa
Protein Length	Full Length
Mol. Weight	43.5kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Catalyzes the NAD-dependent oxidation of all-trans-retinol, alcohol, and omega-hydroxy fatty acids and their derivatives. Oxidizes preferentially all trans-retinol, all-trans-4-hydroxyretinol, 9-cis-retinol, 2-hexenol, and long chain omega-hydroxy fatty acids such as juniperic acid. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and aldehydes and their derivatives. Reduces preferentially all trans-retinal, all-trans-4-oxoretinal and hexanal. Catalyzes in the oxidative direction with higher efficiency. Therefore may participate in retinoid metabolism, fatty acid omega-oxidation, and elimination of cytotoxic aldehydes produced by lipid peroxidation.
Subcellular Location	Cytoplasm.
Protein Families	Zinc-containing alcohol dehydrogenase family, Class-IV subfamily
Database References	HGNC: 256 OMIM: 600086 KEGG: hsa:131 STRING: 9606.ENSP00000420269 UniGene: Hs.389
Tissue Specificity	Preferentially expressed in stomach.

Gene Functions References

These results suggest that variants near ADH7 may play a role in protection from alcohol dependence in this Mexican American cohort. PMID: 25527893
Transcriptional regulatory elements of ADH7 were identified that effect gene expression. PMID: 24512552
common ADH1-7 variants conferred risk for both schizophrenia in African-Americans and autism in European-Americans. PMID: 23468174
The rs1573496 (ADH7) polymorphism was not associated with colorectal cancer risk overall in Western-European populations. However, the relationship between alcohol and colorectal cancer risk might be modulated by the rs1573496 (ADH7) polymorphism. PMID: 23149980
The current results support the ADH7 A92G SNP as a marker for the risk of SCCHN in non-Hispanic white populations. PMID: 20336794
An association between age at onset of regular alcohol use and a SNP just upstream of ADH7 (rs2654849, p = .003) was observed. PMID: 20158305
ADH7 gene polymorphisms are associated with upper aerodigestive tract cancers. PMID: 19861527
the ADH7 variation may contribute to the genetic component of variation in personality traits, with the risk for SD and personality traits being partially shared. PMID: 17918242
A recombinational hotspot within intron 7 of the ADH7 gene were seen using 25 SNPs; haplotypes associated with inter-individual variation in the early stages of alcohol metabolism were identified. PMID: 17921519
Changes in the activity of class IV ADH in the sera of patients with gastric cancer seems to be caused by release of the isoenzyme from cancer cells. PMID: 18231859
Single Nucleotide Polymorphism in ADH7 gene is associated with upper aerodigestive cancer. PMID: 18500343

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.