Recombinant Human All-trans-retinol dehydrogenase [NAD (ADH1B) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-04341P
Greater than 90% as determined by SDS-PAGE.
Greater than 90% as determined by SDS-PAGE.

Recombinant Human All-trans-retinol dehydrogenase [NAD (ADH1B) Protein (His-SUMO)

Beta LifeScience SKU/CAT #: BLC-04341P
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Product Overview

Description Recombinant Human All-trans-retinol dehydrogenase [NAD (ADH1B) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity Greater than 90% as determined by SDS-PAGE.
Uniprotkb P00325
Target Symbol ADH1B
Synonyms ADH beta subunit; ADH1B; ADH1B_HUMAN; ADH2; Alcohol dehydrogenase 1B; alcohol dehydrogenase 2 (class I); beta polypeptide; Alcohol dehydrogenase 2; Alcohol dehydrogenase subunit beta; Aldehyde reductase; DKFZp686C06125; OTTHUMP00000220192
Species Homo sapiens (Human)
Expression System E.coli
Tag N-6His-SUMO
Target Protein Sequence STAGKVIKCKAAVLWEVKKPFSIEDVEVAPPKAYEVRIKMVAVGICRTDDHVVSGNLVTPLPVILGHEAAGIVESVGEGVTTVKPGDKVIPLFTPQCGKCRVCKNPESNYCLKNDLGNPRGTLQDGTRRFTCRGKPIHHFLGTSTFSQYTVVDENAVAKIDAASPLEKVCLIGCGFSTGYGSAVNVAKVTPGSTCAVFGLGGVGLSAVMGCKAAGAARIIAVDINKDKFAKAKELGATECINPQDYKKPIQEVLKEMTDGGVDFSFEVIGRLDTMMASLLCCHEACGTSVIVGVPPASQNLSINPMLLLTGRTWKGAVYGGFKSKEGIPKLVADFMAKKFSLDALITHVLPFEKINEGFDLLHSGKSIRTVLTF
Expression Range 2-375aa
Protein Length Full Length of Mature Protein
Mol. Weight 55.7kDa
Research Area Metabolism
Form Liquid or Lyophilized powder
Buffer Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Storage 1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function Catalyzes the NAD-dependent oxidation of all-trans-retinol and its derivatives such as all-trans-4-hydroxyretinol and may participate in retinoid metabolism. In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and its derivatives such as all-trans-4-oxoretinal. Catalyzes in the oxidative direction with higher efficiency. Has the same affinity for all-trans-4-hydroxyretinol and all-trans-4-oxoretinal.
Subcellular Location Cytoplasm.
Protein Families Zinc-containing alcohol dehydrogenase family
Database References

HGNC: 250

OMIM: 103720

KEGG: hsa:125

STRING: 9606.ENSP00000306606

UniGene: PMID: 28566767

  • ADH1B rs3811802 and ADH1C rs4147542 significantly modified the alcohol-colon cancer association in women. PMID: 29390059
  • The results of the study do not support our hypothesis that ADH1B and ADH7 genotypes affect blood ethanol, and acetaldehyde concentration. PMID: 28731573
  • ADH1B*2/*2 genotype and amount of alcohol consumption increase the risk of atrial fibrillation recurrence after catheter ablation PMID: 28975638
  • ADH1B*3 was not significantly associated with drinking motives or drinking behaviors. However, significant interaction effects of ADH1B*3 with drinking motives on drinking behavior were found; the presence of an ADH1B*3 allele protected against greater drinking quantity among students with high social motives PMID: 28662358
  • the mediation effects of single nucleotide polymorphisms (SNPs) in the ADH1B and ALDH2 genes on hepatocellular carcinoma, were investigated. PMID: 29600553
  • rs17033 of ADH1B and rs671 of ALDH2 SNPs were associated with GC risk and smoking habit may further modify the effect of rs671. Conversely, rs4147536 of ADH1B might have a protective role in our study population. PMID: 29166882
  • Serum triglycerides were positively associated with alcohol consumption in the ADH1B His/His group. PMID: 29176272
  • ADH1B and CDH1 polymorphisms predict prognosis in male patients with non-metastatic laryngeal cancer PMID: 27689323
  • The single nuclear polymorphism rs1229984 in ADH1B results in a gain of function and hence an increase in enzyme activity resulting in the production of excess acetaldehyde. It is found in 19-91% of East Asians but in 0-10% of other populations. PMID: 27575312
  • Alcohol dehydrogenase 1B (ADH1B) catalyzes the oxidation of alcohol to acetaldehyde. PMID: 25828809
  • Men carrying the ADH1B rs1229984 G>A polymorphism consumed significantly less alcohol than non-carriers. PMID: 26644136
  • study demonstrates that ADH1B*3 may protect college students of African descent against alcohol outcomes, although only in low alcohol-facilitating environments. PMID: 28494133
  • In Japanese alcoholic men, ADH1B*1/*1 genotype increased the risk of thrombocytopenia. PMID: 27991683
  • In analysis using the ADH1B and ADH1C genotypes as instrumental variables for alcohol consumption, the hazards ratio for atrial fibrillation per genetic drink per day was 0.79 (0.44-1.41) among men and 0.64 (0.12-3.42) among women PMID: 27071860
  • enhanced Alcohol elimination rate in ADH1B*2 carriers and the increased salivary Acetaldehyde levels in ALDH2*1/*2 carriers among intoxicated alcoholics provide possible mechanisms explaining how each genetic polymorphism a ff ects the risk of alcoholism and upper aerodigestive tract cancer. PMID: 27087413
  • Our findings elucidated the crucial role of multiple genetic variations in ADH1B and ALDH2 as biomarkers of metachronous ESCC. PMID: 27038040
  • Besides the amount and frequency of alcohol drinking, the combination of ALDH2 and ADH1B polymorphisms predicts smoking initiation. This study suggests that alcohol tolerance regulated by ALDH2 and ADH1B polymorphisms is associated with smoking initiation, and facilitates the development of targeted interventions to reduce smoking prevalence. PMID: 28212515
  • This study showed ADH1B appears to affect phenotypic traits via both alcohol-mediated and alcohol-independent effects. PMID: 27187070
  • ADH1B gene Arg47His variant was associated with the decreased esophageal cancer risk. PMID: 27450204
  • Polymorphisms on ADH1B and ALDH2 had significant indirect effects on hepatocellular carcinoma risk, mediated through alcohol drinking PMID: 26827895
  • nucleotide polymorphisms (SNPs) of alcohol dehydrogenase (ADH1B) and aldehyde dehydrogenase 2 (ALDH2) genes encoding enzymes of alcohol and reactive aldehyde metabolism, respectively, are prevalent among East Asians. PMID: 27927211
  • Data suggest that activation of the AhR/ARNT (aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator) signaling by AhR ligands (environmental carcinogens TCDD/3MC/PCB used here) represents novel mechanism for regulating the expression of ADH1B (alcohol dehydrogenase 1B) and other isoenzymes (ADH4, ADH6) in hepatocytes. PMID: 27055685
  • No significant relationship was measured between ADH1B Arg47His and gastric cancer risk; ALDH2 Glu487Lys and CYP4502E1 polymorphisms could be risk factors for the development of gastric cancer in the Chinese population. PMID: 28002588
  • ADH1B rs1229984 and ALDH2 rs671 polymorphisms increased the risk of Alzheimer's disease in all the genetic models. PMID: 27808372
  • ADH1B Arg47His polymorphism, but not the ALDH2 Glu487Lys variation, may influence development of colorectal cancer in the Chinese population. PMID: 27706775
  • No significant association was found between the ADH2 Arg47His polymorphism and risk of Parkinson's disease in the co-dominant, dominant, or recessive models. PMID: 27706693
  • ADH1B polymorphisms influence bladder cancer risk associated with alcohol drinking. PMID: 26992901
  • The faster metabolism of ethanol and acetaldehyde by the ADH1B*2 allele and ALDH2*1/*1 genotype and higher ketosis levels were associated with higher UA levels in alcoholics, while diabetes and the consumption of sake were negative determinants. PMID: 26542604
  • Leukocyte counts of male Japanese alcoholics were affected by ADH1B and ALDH2 genetic variants. PMID: 26917006
  • Initiating moderate red wine consumption at dinner among type 2 diabetes patients does not have a discernable effect on mean 24-h BP. Yet, a modest temporal BP reduction could be documented, and a more pronounced BP-lowering effect is suggested among fast ethanol metabolizers. PMID: 26232779
  • The authors for the first time observed a significant difference in alcohol sensitivity between ADH1B*1/*2 and ADH1B*2/*2 in older men aged 65 and above. PMID: 26825972
  • the combination of genotypes ADH2 * 2, CYP2E1 * 1 combined with genotype homozygous ALDH2 * 1 found in this study could be leading to the population to a potential risk to alcoholism. PMID: 26848198
  • suggested that having the ADH1B*2/*2 genotype caused a positive reaction in subjects with the ALDH2*1/*1 genotype PMID: 25994345
  • Even at early stages of malignant transformation, a significant decrease in ADH1B, ADH3, RDHL, and RALDH1 mRNA levels was observed in 82, 79, 73, and 64% of tumor specimens, respectively PMID: 27239845
  • The presence of the ADH1B*2 allele and the active ALDH2*1/*1 genotype increased the odds ratio for a high TG level. PMID: 26284938
  • Women drinking less alcohol were more likely to carry the rare allele at rs1229984, particularly for consumption before and during pregnancy. PMID: 24065783
  • modulates alcohol consumption [meta-analysis] PMID: 26036284
  • ADH1B polymorphisms may be related to comorbid bipolar II disorder and alcohol dependence. PMID: 26033520
  • ADH1B and ADH1C genetic variations are not related to the development of alcoholism or susceptibility to alcoholic cirrhosis. PMID: 26042511
  • Results show that mutation of this protein confers significant protection against alcohol dependence. PMID: 24735490
  • ADH1B and (ALDH2) polymorphisms in Japanese employees were associated with increased risk for anxiety and depression and alcohol-related disorders. PMID: 25543168
  • a central role for ADH1B in obesity and insulin resistance PMID: 25830378
  • The study explores the association of ADH1B and ADH1C genotype with alcohol consumption and biomarkers of liver function. PMID: 25503943
  • Findings indicate that ALDH2*2, rather than ADH1B2*2, is a causal variant allele for the accumulation of blood acetaldehyde and the resultant facial flushing during low alcohol consumption. PMID: 25365528
  • Results show that different types of both divergent and stabilizing selection processes on ADH1B genotypes were observed in different parts of Asia, but all the data demonstrated a clear positive selection for allele 48His at this locus. PMID: 25019189
  • ADH1B +3170A>G and ADH1C +13044A>G SNPs are associated with an increased risk of head and neck squamous cell carcinoma , and they could be used as biomarkers for the high-risk group of HNSCC in Koreans. PMID: 25874489
  • Studies have shown that A carriers of ADH1B rs1229984 are less likely to drink alcohol. PMID: 24716453
  • High expression level of ADH1B is associated with increased incidence of residual disease in high-grade serous ovarian cancer after surgery. PMID: 24756370
  • ADH1B variants and childhood diversity were associated with alcohol related disorders in African-Americans and Whites in both men and women. PMID: 25410943

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    Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

    Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

    Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

    Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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