Recombinant Human Aldolase B / ALDB Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0133
Recombinant Human Aldolase B / ALDB Protein (GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0133
Collections: Other recombinant proteins, Recombinant proteins
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | GST |
Host Species | Human |
Accession | P05062 |
Synonym | ALDB, ALDO2 |
Background | The aldolase family members involved in metabolism and glycolysis are present in three isoforms: ALDOA,- ALDOB, and ALDOC. Aldolases are differentially expressed in human tissues, and aberrant expression has been observed in several human diseases and cancer types.- Via GATA6, metastatic cells in the liver upregulate the enzyme aldolase B (ALDOB), which enhances fructose metabolism and provides fuel for major pathways of central carbon metabolism during tumor cell proliferation. Targeting ALDOB or reducing dietary fructose significantly reduces liver metastatic growth but has little effect on the primary tumor. Hereditary fructose intolerance (HFI) is an autosomal recessive disorder caused by aldolase B (ALDOB) deficiency resulting in an inability to metabolize fructose. The toxic accumulation of intermediate fructose-1-phosphate causes multiple metabolic disturbances, including postprandial hypoglycemia, lactic acidosis, electrolyte disturbance, and liver/kidney dysfunction. |
Description | A DNA sequence encoding the human ALDOB (P05062) (Ala 2-Tyr 364) was fused with the GST tag at the N-terminus. |
Source | E.coli |
Predicted N Terminal | Met |
AA Sequence | Ala 2-Tyr 364 |
Molecular Weight | The recombinant human ALDOB/GST chimera consists of 597 a.a. and has a predicted molecular mass of 66.5 kDa. It migrates as an approxiamtely 60 KDa band in SDS-PAGE under reducing conditions. |
Purity | >88% as determined by SDS-PAGE |
Endotoxin | Please contact us for more information. |
Bioactivity | Please contact us for detailed information |
Formulation | Lyophilized from sterile PBS, pH 7.5. |
Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
Usage | For Research Use Only |
Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |