Recombinant Human ALDH4A1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-0130
Recombinant Human ALDH4A1 Protein
Beta LifeScience
SKU/CAT #: BLPSN-0130
Catalog No.: BLPSN-0130
Product Overview
| Tag | N/A |
| Host Species | Human |
| Accession | AAH07581.1 |
| Synonym | ALDH4, P5CD, P5CDh |
| Background | ALDH4A1 is a member of the aldehyde dehydrogenase family. Aldehyde dehydrogenase enzymes function in the metabolism of many molecules including certain fats (cholesterol and other fatty acids) and protein building blocks (amino acids). Additional aldehyde dehydrogenase enzymes detoxify external substances, such as alcohol and pollutants, and internal substances, such as toxins that are formed within cells. ALDH4A1 is expressed abundantly in liver followed by skeletal muscle, kidney, heart, brain, placenta, lung and pancreas. It is a mitochondrial matrix NAD-dependent dehydrogenase which catalyzes the second step of the proline degradation pathway, converting pyrroline-5-carboxylate to glutamate. Defects in ALDH4A1 are the cause of hyperprolinemia type 2 (HP-2). HP-2 is characterized by the accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. The disorder may be causally related to neurologic manifestations, including seizures and mental retardation. |
| Description | A DNA sequence encoding the human ALDH4A1 (AAH07581.1) (Lys 25-Gln 563) was expressed and purified with two additional amino acids (Gly & Pro ) at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Gly |
| AA Sequence | Lys 25-Gln 563 |
| Molecular Weight | The secreted recombinant human ALDH4A1 consists of 541 a.a. and predicts a molecular mass of 59.2 KDa. The apparent molecular mass of the protein is approximately 54 KDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.5.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
