Recombinant Human Aldehyde Dehydrogenase Family 1 Member A3 (ALDH1A3) Protein (His-SUMO)

Name: Recombinant Human Aldehyde Dehydrogenase Family 1 Member A3 (ALDH1A3) Protein (His-SUMO)
Brand: Beta LifeScience
SKU: BLC-09226P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Human Aldehyde Dehydrogenase Family 1 Member A3 (ALDH1A3) Protein (His-SUMO) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P47895
Target Symbol	ALDH1A3
Synonyms	Acetaldehyde dehydrogenase 6; AL1A3_HUMAN; Aldehyde dehydrogenase 1A3; Aldehyde dehydrogenase 6; Aldehyde dehydrogenase family 1 member A3; Aldh1a3; ALDH1A6; ALDH6; RALDH 3; Retinaldehyde dehydrogenase 3; V1
Species	Homo sapiens (Human)
Expression System	E.coli
Tag	N-6His-SUMO
Target Protein Sequence	ATANGAVENGQPDRKPPALPRPIRNLEVKFTKIFINNEWHESKSGKKFATCNPSTREQICEVEEGDKPDVDKAVEAAQVAFQRGSPWRRLDALSRGRLLHQLADLVERDRATLAALETMDTGKPFLHAFFIDLEGCIRTLRYFAGWADKIQGKTIPTDDNVVCFTRHEPIGVCGAITPWNFPLLMLVWKLAPALCCGNTMVLKPAEQTPLTALYLGSLIKEAGFPPGVVNIVPGFGPTVGAAISSHPQINKIAFTGSTEVGKLVKEAASRSNLKRVTLELGGKNPCIVCADADLDLAVECAHQGVFFNQGQCCTAASRVFVEEQVYSEFVRRSVEYAKKRPVGDPFDVKTEQGPQIDQKQFDKILELIESGKKEGAKLECGGSAMEDKGLFIKPTVFSEVTDNMRIAKEEIFGPVQPILKFKSIEEVIKRANSTDYGLTAAVFTKNLDKALKLASALESGTVWINCYNALYAQAPFGGFKMSGNGRELGEYALAEYTEVKTVTIKLGDKNP
Expression Range	2-512aa
Protein Length	Full Length of Mature Protein
Mol. Weight	72.0kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	NAD-dependent aldehyde dehydrogenase that catalyzes the formation of retinoic acid. Has high activity with all-trans retinal, and has much lower in vitro activity with acetaldehyde. Required for the biosynthesis of normal levels of retinoic acid in the embryonic ocular and nasal regions; retinoic acid is required for normal embryonic development of the eye and the nasal region.
Subcellular Location	Cytoplasm.
Protein Families	Aldehyde dehydrogenase family
Database References	HGNC: 409 OMIM: 600463 KEGG: hsa:220 STRING: 9606.ENSP00000332256 UniGene: PMID: 30143021 Data indicate that aldehyde dehydrogenase 1A3 (ALDH1A3), that is directly involved in therapy resistance of glioblastoma, is regulated by autophagy during chemotherapy. PMID: 29306018 ALDH1A3 suppression could be one of PPARG tumor suppressive function. This study provides a better understanding of the role of PPARG in lung cancer. PMID: 29873276 Here the authors report the first crystal structure of human ALDH1A3 complexed with NAD(+) and the product all-trans retinoic acid (REA). The tetrameric ALDH1A3 folds into a three domain-based architecture highly conserved along the ALDHs family. The structural analysis revealed two different and coupled conformations for NAD(+) and REA that the authors propose to represent two snapshots along the catalytic cycle. PMID: 27759097 Data show that retinoic acid receptor responder 1 (RARRES1) expression is subtype-dependent and regulated by DNA methylation and the expression of aldehyde dehydrogenase 1A3 (ALDH1A3). PMID: 27286452 ALDH1A3 is a poor prognostic factor for patients with intrahepatic cholangiocarcinoma who underwent hepatectomy and in those with advanced intrahepatic cholangiocarcinoma receiving chemotherapy. PMID: 27076629 high transcription activities of ALDH1A2, ALDH1A3 and ALDH1L1 predicted worsen overall survival in gastric cancer patients PMID: 27015121 Melanoma treatment with the novel irreversible isoform-specific ALDH1 inhibitor [4-dimethylamino-4-methyl-pent-2-ynthioic acid-S methylester] di-methyl-ampal-thio-ester (DIMATE) or depletion of ALDH1A1 and/or ALDH1A3, promoted the accumulation of apoptogenic aldehydes leading to apoptosis and tumor growth inhibition in immunocompetent, immunosuppressed and patient-derived xenograft mouse models. PMID: 28581514 these findings ascribe a novel function for ALDH1A3 in an aggressive glioma stem cells phenotype via the up-regulation of tissue transglutaminase PMID: 28423611 our findings define a FOXD1-ALDH1A3 pathway in controling the clonogenic and tumorigenic potential of mesenchymal glioma stem-like cells in glioblastoma tumors PMID: 27569208 we looked up our single center primary prostate cancer post-operative follow-up data and suggested that the high level ALDH1A3 expression could predict the poor progression-free survival in a 158-patient cohort. We concluded that ALDH1A3, localized in luminal layer in prostate epithelium, is highly expressed in prostate cancer PMID: 28443495 this report brings new information for the phenotype-genotype correlation of ALDH1A3 mutations and raises important questions, especially in terms of genetic counselling given to the patients and their families PMID: 26873617 ALDH1A3 was most relevant to extracellular matrix organization and cell adhesion biological process, and the ability of tumor invasion was suppressed after ALDH1A3 knockdown in vitro. PMID: 26575197 the prevalence of ALDH1A3(+)/CD44(+) tumor cells in breast cancer is significantly associated with worse prognostic factors and favors a poor prognosis PMID: 26687806 Whole-exome sequencing in a South American cohort links ALDH1A3, FOXN1 and RARB/retinoic acid regulation pathways to autism spectrum disorders. PMID: 26352270 we demonstrated that a specific ALDH isoform, namely ALDH1A3, is enriched in chemoresistant mesothelioma cell subpopulations PMID: 25868979 ALDH1A3 induces differential RA signaling in breast cancer cells which affects the rate of breast cancer progression. PMID: 25106087 ALDH1A3 is a target of miR-187 in human prostate cancer. PMID: 25969992 Increased hypermethylation of ALDH1A3 is associated with Glioblastoma. PMID: 25684492 Among unique genes up-regulated by Y15 in Lovo-1 and K1 resistant cells, a stem cell marker-ALDH1A3-was detected to be up-regulated >twofold. PMID: 25656374 High ALDH1A3 expression is associated with non-small cell lung cancer. PMID: 24907115 Mutations in ALDH1A3 represent a frequent cause of microphthalmia/anophthalmia in consanguineous families. PMID: 24777706 At the transcript level, the cisplatin + DEAB-resistant cells showed upregulated mRNA expression levels for ALDH1A2, ALDH1A3 isozymes and CD44 indicating the involvement of these markers in conferring chemoresistance PMID: 24884875 Nine individuals, from one inbred kindred, segregating isolated A/M with orbital cysts were homozygous for a missense mutation in the gene encoding the A3 isoform of the ALDH1A3. PMID: 23881059 Novel ALDH1A3 mutations identified in the present study confirm the pivotal role of ALDH1A3 in human eye development. PMID: 24568872 ALDH1A3 may be upregulated in pancreatic cancer PMID: 24053169 ALDH1A3 expression determines the stem cell-like cellular states in EGFR-mutant non-small cell lung cancer; a novel mechanism of acquired resistance to erlotinib can be targeted with the natural polyphenol silibinin. PMID: 24047698 Our data support the very recent and independent identification of ALDH1A3 as a disease gene in microphthalmia. PMID: 23646827 Results show that ALDH1A3 loss of function causes anophthalmia and aberrant eye development in humans and in animal model systems. PMID: 23591992 The effects of phenotypic and morphological alterations by DEAB on oral keratinocytes are mainly consequent to the inhibition of ALDH1A3 activity. PMID: 23250514 The methylation status of Homeobox A9 (HOXA9), ISL LIM homeobox 1 (ISL1) and Aldehyde dehydrogenase 1 family, member A3 (ALDH1A3) was significantly associated with decreased gene expression levels PMID: 23436614 two subtypes of GSCs, harboring distinct metabolic signaling pathways, represent intertumoral glioma heterogeneity and highlight previously unidentified roles of ALDH1A3-associated signaling that promotes aberrant proliferation of Mes HGGs and GSCs. PMID: 23650391 Homozygosity for one splice-site and two missense mutations in the gene encoding the A3 isoform of the aldehyde dehydrogenase 1 (ALDH1A3) in three consanguineous families, is reported. PMID: 23312594 ALDH1A3 transcripts are detected at high levels in luminal progenitor-enriched fractions obtained from short-term adherent cultures of freshly isolated normal human mammary cells. PMID: 22131125 ALDH1A3 expression in patient breast tumors correlates significantly with tumor grade, metastasis, and cancer stage PMID: 21280157 these results indicate that the regulation of the retinoic acid metabolism in the epidermis involves transcriptional activation of ALDH1A3, possibly representing a positive feedback loop, which enhances the effect of exogenous retinoic acid. PMID: 20709019 play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. PMID: 7698756

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

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