Recombinant Human alanyl-tRNA synthetase / CMT2N Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0125
Recombinant Human alanyl-tRNA synthetase / CMT2N Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0125
Catalog No.: BLPSN-0125
Product Overview
| Tag | His |
| Host Species | Human |
| Synonym | CMT2N |
| Background | Alanyl-tRNA synthetase (AARS) belongs to the family of ligases, specifically those forming carbon-oxygen bonds in aminoacyl-tRNA and related compounds. This enzyme participates in alanine and aspartate metabolism and aminoacyl-tRNA biosynthesis. Alanyl-tRNA synthetase (AlaRS) catalyzes synthesis of Ala-tRNA (Ala) and hydrolysis of mis-acylated Ser- and Gly-tRNA (Ala) at 2 different catalytic sites. Their role is not confined to catalyze the attachment of amino acids to transfer RNAs and thereby establish the rules of genetic code by virtue of matching the nucleotide triplet of anticodon with cognate amino acid. Under apoptotic conditions in cell culture, the full-length enzyme is secreted, and the two cytokine activities can be generated by leukocyte elastase, an extracellular protease. Secretion of this tRNA synthetase may contribute to apoptosis both by arresting translation and producing needed cytokines. This protein could be an attractive target of drugs against bacterial, fungal and parasitic infections. |
| Description | A DNA sequence encoding the human AARS (NP_001596.2) (Met1-Asn968) was expressed with a His tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | His |
| AA Sequence | Met1-Asn968 |
| Molecular Weight | The recombinant human AARS consists of 987 a.a. and predicts a molecular mass of 109.2 kDa. |
| Purity | >85% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile 20 mM PB, 500 mM NaCl, pH 7.0, 20 % glycerol.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
