Recombinant Human AK2 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0113
Recombinant Human AK2 Protein (His & GST Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0113
Catalog No.: BLPSN-0113
Product Overview
| Tag | His&GST |
| Host Species | Human |
| Accession | P54819 |
| Synonym | ADK2, AK2 |
| Background | Adenylate kinase 2 (AK2) belongs to the Adenylate kinase family that contains three isozymes: AK1, AK2 and AK3. Adenylate kinases are involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. Adenylate kinase2 (AK2) is expressed in mitochondrial intermembrane space. It may play a role in apoptosis. It has been demonstrated that in apoptotic cells AK2 was translocated into the cytosol concomitantly with cytochronme C. Mutations in this gene are the cause of reticular dysgenesis. These mutations result in absent or strongly decreased protein expression. It has been also established that AK2 is specifically expressed in the stria vascularis region of the inner ear, which provides an explanation of the sensorineural deafness in these individuals. |
| Description | A DNA sequence encoding the human AK2 isoform 1 (P54819-1) (Met 1-Ile 239) was fused with the N-terminal His-tagged GST tag at the N-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met |
| AA Sequence | Met 1-Ile 239 |
| Molecular Weight | The recombinant human AK2/GST chimera consists of 476 a.a. and has a calculated molecular mass of 54.3 kDa. It migrates as an approximately 50 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >95% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly, 3mM DTT. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
