Recombinant Human AHSP Protein

Beta LifeScience SKU/CAT #: BLPSN-0111
Recombinant Human AHSP Protein

Recombinant Human AHSP Protein

Beta LifeScience SKU/CAT #: BLPSN-0111
Catalog No.: BLPSN-0111

Product Overview

Tag N/A
Host Species Human
Accession Q9NZD4
Synonym EDRF, ERAF
Background AHSP, also known as ERAF, is a conserved mammalian erythroid protein which belongs to the AHSP family. It is expressed in blood and bone marrow. AHSP facilitates the production of Hemoglobin A by stabilizing free alpha-globin. It rapidly binds to ferrous alpha with association (k'(AHSP)) and dissociation (k(AHSP)) rate constants of ≈1 μm(-1) s(-1) and .2 s(-1), respectively, at pH 7.4 at 22 °C. A small slow phase was observed when AHSP binds to excess ferrous alphaCO. This slow phase appears to be due to cis to trans prolyl isomerization of the Asp(29)-Pro(3) peptide bond in wild-type AHSP because it was absent when alphaCO was mixed with P3A and P3W AHSP, which are fixed in the trans conformation. This slow phase was also absent when met(Fe(3+))-alpha reacted with wild-type AHSP, suggesting that met-alpha is capable of rapidly binding to either Pro(3) conformer. Both wild-type and Pro(3)-substituted AHSPs drive the formation of a met-alpha hemichrome conformation following binding to either met- or oxy(Fe(2+))-alpha. The dissociation rate of the met-alpha-·AHSP complex (k(AHSP) ≈ .2 s(-1)) is ~1-fold slower than that for ferrous alpha-·AHSP complexes, resulting in a much higher affinity of AHSP for met-alpha. Thus, in vivo, AHSP acts as a molecular chaperone by rapidly binding and stabilizing met-alpha hemichrome folding intermediates. The low rate of met-alpha dissociation also allows AHSP to have a quality control function by kinetically trapping ferric alpha and preventing its incorporation into less stable mixed valence Hemoglobin A tetramers. Reduction of AHSP-bound met-alpha allows more rapid release to beta subunits to form stable fully, reduced hemoglobin dimers and tetramers.
Description A DNA sequence encoding human ERAF (Q9NZD4) (Met1-Ser102) was expressed.
Source E.coli
Predicted N Terminal Met
AA Sequence Met1-Ser102
Molecular Weight The recombinant human ERAF consists of 102 a.a. and predicts a molecular mass of 11.8 KDa. It migrates as an approximately 12 KDa band in SDS-PAGE under reducing conditions.
Purity >90% as determined by SDS-PAGE
Endotoxin Please contact us for more information.
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Recently viewed