Recombinant Human ACVR1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0066
Recombinant Human ACVR1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0066
Catalog No.: BLPSN-0066
Product Overview
| Tag | His |
| Host Species | Human |
| Accession | Q04771 |
| Synonym | ACTRI, ACVR1A, ACVRLK2, ALK2, FOP, SKR1, TSRI |
| Background | ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP). |
| Description | A DNA sequence encoding the extracellular domain (Met 1-Val 124) of human ALK2 (Q04771) (Met 1-Val 124) was fused with a His tag at the C-terminus. |
| Source | Baculovirus-Insect Cells |
| Predicted N Terminal | Met 21 |
| AA Sequence | Met 1-Val 124 |
| Molecular Weight | The secreted recombinant human ALK2 consists of 114 a.a. and has a calculated molecular mass of 12.8KDa. It migrates as an approximately 17 kDa band in SDS-PAGE under reducing conditions. |
| Purity | >93% as determined by SDS-PAGE |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
| Formulation | Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
