Name: Recombinant Human Acid sphingomyelinase / ASM Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0051
Availability: InStock

Recombinant Human Acid sphingomyelinase / ASM Protein (His Tag)

Catalog No.: BLPSN-0051

Product Overview

Tag	His
Host Species	Human
Accession	BAD93012.1
Synonym	ASM, ASMASE, NPD
Background	Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.
Description	A DNA sequence encoding the human SMPD1 (BAD93012.1) (Met1-Pro628) was expressed with a His tag at the C-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	Leu 47
AA Sequence	Met1-Pro628
Molecular Weight	The recombinant human SMPD1 consists of 593 a.a. and predicts a molecular mass of 66.3 kDa.
Purity	>95% as determined by SDS-PAGE.
Endotoxin	< 1.0 EU per μg protein as determined by the LAL method.
Bioactivity	Please contact us for detailed information
Formulation	Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 25 % glycerol, pH 7.5..
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.