Name: Recombinant Human Acid sphingomyelinase / ASM Protein (His Tag)
Brand: Beta LifeScience
SKU: BLPSN-0049
Availability: InStock

Recombinant Human Acid sphingomyelinase / ASM Protein (His Tag)

Catalog No.: BLPSN-0049

Product Overview

Tag	His
Host Species	Human
Accession	NP_000534.3
Synonym	ASM, ASMASE, NPD
Background	Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86% aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.
Description	A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a His tag at the C-terminus.
Source	Baculovirus-Insect Cells
Predicted N Terminal	His 62
AA Sequence	Met 1-Cys 631
Molecular Weight	The secreted recombinant human SMPD1 consists of 518 a.a. and predicts a molecular mass of 65 kDa as estimated by SDS-PAGE under reducing conditions.
Purity	>94% as determined by SDS-PAGE
Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method
Bioactivity	Measured by its ability to cleave. 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is >1,000 pmol/min/ug.
Formulation	Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 0.1% OGP, 10% glycerol.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.