Recombinant Human Acid Phosphatase / HAAP Protein (GST Tag)

Name: Recombinant Human Acid Phosphatase / HAAP Protein (GST Tag)
Brand: Beta LifeScience
SKU: BLPSN-0048
Availability: InStock

Collections: Other recombinant proteins, Recombinant proteins

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Product Overview

Tag	GST
Host Species	Human
Accession	AAI06012.1
Synonym	HAAP
Background	The low molecular weight phosphotyrosine phosphatase (LMW-PTP), also known as Acid phosphatase 1 (ACP1), belongs to the low molecular weight phosphotyrosine protein phosphatase family are involved in the regulation of important physiological functions, including stress resistance and synthesis of the polysaccharide capsule. ACP1/LMW-PTP is an enzyme involved in platelet-derived growth factor-induced mitogenesis and cytoskeleton rearrangement. LMW-PTP is able to specifically bind and dephosphorylate activated PDGF receptor, thus modulating PDGF-induced mitogenesis. In vitro, LMW-PTP was found to efficiently dephosphorylate activated FcgammaRIIA and LAT, but not Syk or phospholipase Cgamma2. The overexpression of LMW-PTP inhibited activation of Syk downstream of FcgammaRIIA and reduced intracellular Ca(2+) mobilization. It been demonstrated that LMW-PTP is responsible for FcgammaRIIA dephosphorylation, and is implicated in the down-regulation of cell activation mediated by this ITAM-bearing immunoreceptor. In addition, ACP1 is a highly polymorphic phosphatase that is especially abundant in the central nervous system and is known to be involved in several signal transduction pathways.
Description	A DNA sequence encoding human ACP1 (AAI06012.1) (Met 1-His 158) was fused with the GST tag at the N-terminus.
Source	E.coli
Predicted N Terminal	Met
AA Sequence	Met 1-His 158
Molecular Weight	The recombinant human ACP1/GST chimera consists of 384 a.a. and predicts a molecular mass of 44.3 kDa. The apparent molecular mass of rh ACP1 is approximately 40 kDa in SDS-PAGE under reducing conditions.
Purity	>88% as determined by SDS-PAGE
Endotoxin	Please contact us for more information.
Bioactivity	Measured by its ability to cleave a substrate, pNitrophenyl phosphate (pNPP). The specific activity is >65,000 pmol/min/ug.
Formulation	Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 8.0.
Stability	The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage	For Research Use Only
Storage	Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Target Details

Target Function	Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Isoform 3 does not possess phosphatase activity.
Subcellular Location	Cytoplasm.
Protein Families	Low molecular weight phosphotyrosine protein phosphatase family
Database References	HGNC: 122 OMIM: 171500 KEGG: hsa:52 STRING: 9606.ENSP00000272065 UniGene: Hs.558296
Tissue Specificity	T-lymphocytes express only isoform 2.

Gene Functions References

ACP1 may have an important role in regulation of the multiple systems associated with suicide. PMID: 28668716
Conditioned medium from MDA-MB-231 breast cancer cells with total knockdown of LMW-PTP, but not of slow isoform LMW-PTP, significantly reduced osteoclast differentiation of RAW 264.7 cells. PMID: 29187443
results confirm the role of TPH1, TPH2, 5HT2A, CRHR1 and ACP1 variants in the risk of suicidal behavior. PMID: 27479537
These data support that PTPN22 1858C/T, PTPRJ 2965C/G and 1176 A/C polymorphisms and ACP1 A, B and C alleles are not associated with a higher risk of immune thrombocytopenia P in adults. PMID: 27309885
The LMW-PTP slow isoform can be an important protein in bone metastatic disease, with a fundamental role in the interplay between tumor cells and osteoclasts, through the regulation of Src activity and IL-8 secretion. PMID: 27127127
Overexpression of LMWPTP in prostate cancer confers a malignant phenotype with worse clinical outcome. PMID: 26159288
ADA6, PTPN22 and ACP1 are involved in immune reactions: since endometriosis has an autoimmune component. PMID: 26216523
LMPTP expression increases in end-stage heart failure in humans PMID: 26213100
Suggest that hypermethylation of ACP1, BMP4, and TSPYL5 are common events in HCC and could be used as potentially detectable biomarkers in HCC tissues. PMID: 26386860
the present study found that the ACP1*C allele, previously associated with an increased vulnerability to infectious/parasitic diseases may also be able to shape behavioral immune defenses by interaction with the level of E. PMID: 24933463
Association of an increased risk of coronary artery disease, especially in females, with ACP1 polymorphisms. PMID: 25846885
ACP1 knockdown attenuates effects of osmotic stress in HaCaT cells, mainly in the status of Src kinase, Rac and STAT5 phosphorylation and activity. PMID: 25781955
The rs3828329 of ACP1 gene is also a risk factor of CAD in Han Chinese females aged 65 years and older. PMID: 25123136
ACP1 polymorphism is associated with type 1 diabetes mellitus. PMID: 25125338
In ACP1 *B/*C genotype, which shows the highest enzymatic activity, spermatic concentration is significantly lower and atypical spermatozoa are significantly more frequent as compared to other ACP1 genotypes. PMID: 23278455
In Class 3 people the combination of high ACP f-isozyme concentration and the ADA*2 allele, lowers the rate of glycolysis that may reduce the amount of metabolic calories and activates Sirtuin genes that protect cells against age-related diseases. PMID: 23959645
certain genotypes of ACP1 associated with high phosphatase activity may increase the T-cell response to PF4-heparin complexes, with higher levels of circulating antibodies PMID: 23621699
A significant increase of PTPN22 *T allele in endometriosis is observed in women carrying ACP1*C allele, in women carrying p53 codon 72 *Pro allele PMID: 23453606
clarification of the importance of ACP1 in carcinogenesis through the analysis of LMW-PTP interaction with different substrates [review] PMID: 23584899
The data suggest an interaction between p53 codon 72 and ACP wherein a positive effect of the p53 *Pro allele on susceptibility to coronary artery disease occurs. PMID: 23197232
mRNA expression of the slow isoform was increased in breast cancer and that of the fast isoform was reduced in breast cancer. PMID: 23645747
balanced redox-state is required for VEGF to facilitate reversible S-glutathionylation of LMW-PTP, FAK activation and endothelial cell migration PMID: 22854047
lack of association with inflammatory bowel disease in Spanish patients PMID: 22428720
There is a significant association between acid phosphatase locus 1 (ACP(1) and cancer grade, mainly due to ACP(1) genotypes carrying the *C allele that are much less represented in patients with low grade when compared with those with high grade. PMID: 22692348
the ACP1 protein is a tyrosine phosphatase that influences Wnt signaling, a pathway regulated by lithium, making ACP1 a functional candidate for involvement in the phenotype. PMID: 21423239
Data indicate that ACP1 rs11553742*T with increased susceptibility in systemic lupus erythematosus (SLE) patients. PMID: 22064183
Gain of the telomeric region 2p25.3 harboring the ACP1 gene is common in CLL (25%, 44 of 178 cases). PMID: 22035742
the ACP1*C allele influences the risk of cardiovascular disease events in patients with rheumatoid arthritis. PMID: 21767392
Although both variants dephosphorylate the EPHA2 receptor, the rate and specificity of dephosphorylation for specific tyrosines are different for ACP1 and human cytoplasmic protein tyrosine phosphatase-B. PMID: 21538645
investigated hypothesis that favism is caused by toxic Vicia faba substances, which in some ACP1 phenotypes cause increased phosphorylation and thus increased glycolysis, with strong reduction in reduced glutathione production, resulting in hemolysis PMID: 21644204
In overweight women (BMI > 25), the proportion of low activity ACP1 phenotypes is much lower in type 1 diabetes than in gestational diabetes and in healthy females. PMID: 19855922
the correlation between blood glucose and glycated Hb in relation to AK1 and ACP1 polymorphism was studied. PMID: 20152999
Type 1 diabetes subjects show a highly significant increase of ACP1*A/ADA1*2 gametic type compared with healthy subjects from the same population (P = 0.003). PMID: 20805743
association with coronary artery disease evident only in diabetic subjects and dependent on female gender PMID: 20581655
A significant interaction between ACP1 and ADA1 concerning susceptibility to type 1 diabetes, was revealed. PMID: 19789510
ACP1 is associated with allergy PMID: 12100313
Association of the ACP1 genotype with metabolic parameters upon initial diagnosis of type 1 diabetes. PMID: 12640337
LMW-PTP has a role in immunological synapse establishment and stabilization through the negative control of FAK activity and of cell surface receptor redistribution PMID: 12815062
The analysis involved the data on nine polymorphic codominant loci: HP, GC, TF, PI, PGM1, GLO1, C3, ACP1, and ESD. The loci were selected by significance of differences in genotype frequencies between tuberculosis patients and healthy controls PMID: 12942785
We show that the association of STAT5 and LMW-PTP does not exclusively involve the phosphatase active site and phosphotyrosine residue of STAT5. PMID: 14637146
acid phosphatase 1 contributes to the clinical manifestations of type 2 diabetes and probably also have a marginal influence on susceptibility to the disease PMID: 15281007
complex interaction among maternal age, sex of infant and ACP1 concerning age at diagnosis of diabetes PMID: 15586390
Finds ACP1*C is a recessively deleterious allele that reduces viability during early life stages and is not maintained by overdominant selection in European populations. PMID: 15974295
Significant correlations between LMW-PTP overexpression and the most common clinical-pathological features of cancers exist. In colon cancer and neuroblastoma increased total LMW-PTP mRNA expression correlates with unfavourable outcome. PMID: 16036221
Crystal structure of the second human low molecular weight PTPase isoenzyme provides the opportunity to examine the structural basis of different substrate and inhibitor/activator responses. PMID: 16253994
mother/newborn pairs: ACP1 (acid phosphatase 1)distribution has fewer pairs with maternal low ACP1 S isoform and infant high S isoform concentration;Recurrent spontaneous abortion couples show wife low S isoform and husband high S isoform concentration PMID: 16762482
GRX plays an important role in PDGF-BB-dependent cell proliferation by regulating the redox state of LMW-PTP PMID: 16893901
Among newborns carrying the ACP1C allele there is an increase of Sex Ratio among the offspring of smoking mothers relative to non-smoking mothers. PMID: 16973312
Human recombinant LMWPTP-A displayed an RN5Pase activity that was higher than its tyrosine phosphatase activity, indicating that this phosphatase may participate in protein deglycation, a new form of protein repair. PMID: 17472574
results suggest a cooperative effect of ADA and ACP1 genetic polymorphism on the susceptibility to repeated spontaneous abortion and to some of its clinical characteristics PMID: 17565542

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