Recombinant GST Protein
Beta LifeScience
SKU/CAT #: BL-1041SG
Recombinant GST Protein
Beta LifeScience
SKU/CAT #: BL-1041SG
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Tag | N/A |
Accession | U13850 |
Background | GST or glutathione S-transferase family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are used to create the so-called GST gene fusion system (1). These proteins are capable of multiple reactions with a multitude of substrates. GST tag has the size of 220 amino acids, which is relatively large compared to other tags like the myc- or the FLAG-tag that are quite small. GST can be used to purify and detect proteins of interest. In a GST gene fusion system, an expression vector consists of the gene sequence encoding the protein of interest and GST sequence alongside. After induction, the fusion proteins can be purified using affinity chromatography via its high affinity for glutathione (1). |
Description | Recombinant artificial sequences GST (1-226) was produced in E. coli system and purified on glutathione column. |
Source | E.coli |
AA Sequence | 1-226a.a. |
Molecular Weight | ~25 kDa |
Purity | For specific purity information on a given lot, see related COA. |
Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method |
Formulation | Recombinant protein is supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF and 25% glycerol. |
Stability | The recombinant protein is stable for up to 12 months at -70°C |
Usage | For Research Use Only |
Storage | Recombinant GST Protein should be stored should be stored at < -70°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |