Recombinant E.Coli Atp-Dependent Clp Protease Adapter Protein Clps (CLPS)

Name: Recombinant E.Coli Atp-Dependent Clp Protease Adapter Protein Clps (CLPS)
Brand: Beta LifeScience
SKU: BLC-02639P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant E.Coli Atp-Dependent Clp Protease Adapter Protein Clps (CLPS) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P0A8Q6
Target Symbol	CLPS
Synonyms	clpS; yljA; b0881; JW0865ATP-dependent Clp protease adapter protein ClpS
Species	Escherichia coli (strain K12)
Expression System	E.coli
Tag	N-His&C-Myc/N-His-SUMO&C-Myc/N-His-B2M&C-Myc/N-His-GST&C-Myc/N-His/N-His-SUMO/N-His-B2M/N-His-GST/N-GST/Tag-Free
Target Protein Sequence	MGKTNDWLDF DQLAEEKVRD ALKPPSMYKV ILVNDDYTPM EFVIDVLQKF FSYDVERATQ LMLAVHYQGK AICGVFTAEV AETKVAMVNK YARENEHPLL CTLEKA
Expression Range	1-106
Protein Length	full length protein
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Involved in the modulation of the specificity of the ClpAP-mediated ATP-dependent protein degradation.
Protein Families	ClpS family
Database References	KEGG: ecj:JW0865 STRING: 316385.ECDH10B_0951

Gene Functions References

Between the halves of the clpA pseudo-dimer is a large flexible acidic loop that becomes better ordered upon binding of the small adaptor protein, ClpS PMID: 15037248
the ClpAP-specific adaptor, ClpS, is essential for degradation of N-end rule substrates by ClpAP PMID: 16467841
ClpAP recognizes N-end rule substrates directly, whereas ClpS modulates this degradation pathway. PMID: 17322400
ClpS is an integral and essential component of the N-end rule pathway. PMID: 19317833
The data suggest that ClpS has been optimized for the binding and delivery of N-degrons containing an N-terminal Phe or Leu. PMID: 19373253
ClpS selectively binds N-terminal destabilizing residues and targets them for degradation by the ClpAP complex resulting in carefully regulated proteolysis PMID: 16467841

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.