Recombinant Drosophila Melanogaster Protein Bottleneck (BNK) Protein (His)

Name: Recombinant Drosophila Melanogaster Protein Bottleneck (BNK) Protein (His)
Brand: Beta LifeScience
SKU: BLC-09463P
Availability: InStock

Greater than 90% as determined by SDS-PAGE.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Description	Recombinant Drosophila Melanogaster Protein Bottleneck (BNK) Protein (His) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 90% as determined by SDS-PAGE.
Uniprotkb	P40794
Target Symbol	BNK
Synonyms	bnk; CG1480; Protein bottleneck
Species	Drosophila melanogaster (Fruit fly)
Expression System	E.coli
Tag	N-6His
Target Protein Sequence	MSISTFNFQFYNYKLSPSSPGFGSTASRSSSSCISELEMDIDEDMSAQPTITSTPKPRFTSQLAVELAKTEPGNGISPLRPKLHTAQKRWSMELREKVLEMSKRNNGQEKPQTARQQEQRQPQEQPLQQEELQHQQQEPTVTDKINFFNKLTNTFESGFSKLMPQASSTNNRFIAMLRTARPQNVATTTANSSTANSFLGSDHSLSGSVTGQVPPPKPKRLSATTAQFATPHVPAMGVGKGGSQRKCSLRRKPSMDKSRATISRQSSSASVRTQNHAIMEDLSLVVPVRLRIAEYEQRISMSA
Expression Range	1-303aa
Protein Length	Full Length
Mol. Weight	37.6kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Acts as a regulator of the microfilament network governing cellularization of the embryo. Determines the timing of a key conformational transition in the cortical microfilament network: the proper coordination of membrane invagination and basal closure of the cells. To do this, bnk possibly physically links neighboring contractile units of the early cycle 14 microfilament network in a manner that prevents basal constriction until the proper stage has been reached. Bnk together with nullo and Sry-alpha may provide auxiliary functions, by acting both to stabilize a large and dynamic microfilament structure and regulate its functions.
Subcellular Location	Cytoplasm, cytoskeleton. Note=Colocalizes with the structural transitions in the microfilament network during cellularization. Association is observed only when the network assumes an aligned and tightly apposed hexagonal configuration.
Database References	KEGG: dme:Dmel_CG1480 STRING: 7227.FBpp0085116 UniGene: Dm.1899
Tissue Specificity	Restricted to the blastoderm.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.