Recombinant Cynomolgus TPSAB1 protein

Name: Recombinant Cynomolgus TPSAB1 protein
Brand: Beta LifeScience
SKU: BLK-00935P-100UG
Availability: InStock

Cynomolgus TPSAB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: All products, High-quality recombinant proteins, Other recombinant proteins

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Product Overview

Description	Recombinant Cynomolgus TPSAB1 protein is expressed from E.coli with His tag at the N-Terminus.It contains Ile38-Pro282.
Purity	> 95% as determined by Tris-Bis PAGE
Accession	XP_005590891.2
Target Symbol	TPSAB1
Species	Cynomolgus
Expression System	E. coli
Tag	N-His
Expression Range	Ile38-Pro282
Mol. Weight	The protein has a predicted MW of 28.57kDa. The protein migrates to 30-33kDa based on Tris-Bis PAGE result.
Form	Liquid
Formulation	Supplied as 0.22um filtered solution in 20mM Tris, 250mM NaCl, 2mM CaCl₂, 10% Glycerol (pH 8.0).
Endotoxin	Less than 1EU per ug by the LAL method.
Storage	Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	Shipped with dry ice.
Gene Background	TPSAB1, namely tryptase alpha/beta-1, is a serine protease with trypsinlike activity. TPSAB1 is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It may play a role in innate immunity. Diseases associated with TPSAB1 include mastocytosis and systemic mastocytosis.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.