Recombinant Cynomolgus Angiopoietin-1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0189
Recombinant Cynomolgus Angiopoietin-1 Protein (His Tag)

Recombinant Cynomolgus Angiopoietin-1 Protein (His Tag)

Beta LifeScience SKU/CAT #: BLPSN-0189
Catalog No.: BLPSN-0189

Product Overview

Tag His
Host Species Cynomolgus
Accession XP_005563980.1
Background The angiopoietin (ANGPT)-TIE2/TEK signaling pathway is essential for blood and lymphatic vascular homeostasis. ANGPT1 is a potent TIE2 activator, whereas ANGPT2 functions as a context-dependent agonist/antagonist. In disease, ANGPT2-mediated inhibition of TIE2 in blood vessels is linked to vascular leak, inflammation, and metastasis. Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm's canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. A role for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development.
Description A DNA sequence encoding the cynomolgus ANGPT1 (XP_005563980.1) (Arg277-Phe498) was expressed with a His tag at the N-terminus.
Source HEK293
Predicted N Terminal His
AA Sequence Arg277-Phe498
Molecular Weight The recombinant cynomolgus ANGPT1 consists 241 a.a. and predicts a molecular mass of 27.9 kDa.
Purity >95% as determined by SDS-PAGE.
Endotoxin < 1.0 EU per μg protein as determined by the LAL method.
Bioactivity Please contact us for detailed information
Formulation Lyophilized from sterile PBS, pH 7.4..
Stability The recombinant proteins are stable for up to 1 year from date of receipt at -70°C.
Usage For Research Use Only
Storage Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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