Recombinant Cynomolgus Angiopoietin-1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0189
Recombinant Cynomolgus Angiopoietin-1 Protein (His Tag)
Beta LifeScience
SKU/CAT #: BLPSN-0189
Catalog No.: BLPSN-0189
Product Overview
| Tag | His |
| Host Species | Cynomolgus |
| Accession | XP_005563980.1 |
| Background | The angiopoietin (ANGPT)-TIE2/TEK signaling pathway is essential for blood and lymphatic vascular homeostasis. ANGPT1 is a potent TIE2 activator, whereas ANGPT2 functions as a context-dependent agonist/antagonist. In disease, ANGPT2-mediated inhibition of TIE2 in blood vessels is linked to vascular leak, inflammation, and metastasis. Primary congenital glaucoma (PCG) is a leading cause of blindness in children worldwide and is caused by developmental defects in 2 aqueous humor outflow structures, Schlemm's canal (SC) and the trabecular meshwork. We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. A role for the major ANGPT ligands in the development of the aqueous outflow pathway. We determined that ANGPT1 is essential for SC development, and that Angpt1-knockout mice form a severely hypomorphic canal with elevated intraocular pressure. By linking ANGPT1 with PCG, these results highlight the importance of ANGPT/TEK signaling in glaucoma pathogenesis and identify a candidate target for therapeutic development. |
| Description | A DNA sequence encoding the cynomolgus ANGPT1 (XP_005563980.1) (Arg277-Phe498) was expressed with a His tag at the N-terminus. |
| Source | HEK293 |
| Predicted N Terminal | His |
| AA Sequence | Arg277-Phe498 |
| Molecular Weight | The recombinant cynomolgus ANGPT1 consists 241 a.a. and predicts a molecular mass of 27.9 kDa. |
| Purity | >95% as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg protein as determined by the LAL method. |
| Bioactivity | Please contact us for detailed information |
| Formulation | Lyophilized from sterile PBS, pH 7.4.. |
| Stability | The recombinant proteins are stable for up to 1 year from date of receipt at -70°C. |
| Usage | For Research Use Only |
| Storage | Store the protein under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
