Recombinant Bovine Alpha-S2-Casein (CSN1S2) Protein (His-KSI)

Name: Recombinant Bovine Alpha-S2-Casein (CSN1S2) Protein (His-KSI)
Brand: Beta LifeScience
SKU: BLC-01646P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Description	Recombinant Bovine Alpha-S2-Casein (CSN1S2) Protein (His-KSI) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	P02663
Target Symbol	CSN1S2
Synonyms	Casocidin-1;Casocidin-I
Species	Bos taurus (Bovine)
Expression System	E.coli
Tag	N-6His-KSI
Target Protein Sequence	KNTMEHVSSSEESIISQETYKQEKNMAINPSKENLCSTFCKEVVRNANEEEYSIGSSSEESAEVATEEVKITVDDKHYQKALNEINQFYQKFPQYLQYLYQGPIVLNPWDQVKRNAVPITPTLNREQLSTSEENSKKTVDMESTEVFTKKTKLTEEEKNRLNFLKKISQRYQKFALPQYLKTVYQHQKAMKPWIQPKTKVIPYVRYL
Expression Range	16-222aa
Protein Length	Full Length of Mature Protein
Mol. Weight	39.7 kDa
Research Area	Others
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Important role in the capacity of milk to transport calcium phosphate.; Casocidin-I inhibits the growth of E.coli and S.carnosus.
Subcellular Location	Secreted.
Protein Families	Alpha-casein family
Database References	KEGG: bta:282209 STRING: 9913.ENSBTAP00000006590 UniGene: Bt.57616
Tissue Specificity	Mammary gland specific. Secreted in milk.

Gene Functions References

Translation attenuation via 3' terminal codon usage in bovine mRNA is responsible for the difference in alpha-S2- and beta-casein profile in milk. PMID: 25826667
Data indicate that the pepsin-pancreatin digest of alphaS-Casein exhibited anxiolytic-like activity. PMID: 23580614
Alpha-S2-casein was less effective at preventing fibril formation by modified kappa-casein, whereas alpha- and alpha-S1 casein were comparably potent inhibitors. PMID: 21457703
Single strand conformation polymorphism (SSCP) is a particularly useful technique because it allows discrimination of alleles, including zebu-specific alleles at the CSN2 locus. PMID: 15747725
study reports the SNP and AA change that distinguishes the B allele from other alleles at the CSN1S2 locus PMID: 17582136
Substitution effects of paternal CSN2alpha(s1) allele versus CSN2alpha(s2) allele on protein yield deviations (YDs) are significant, whereas their effects on milk and fat YDs are not. PMID: 18263971
bovine milk alpha s2-casein readily forms fibrils in vitro. Upon incubation at neutral pH & 37 degrees C, spherical particles typical of alpha s2-casein rapidly converted to twisted, ribbon-like fibrils approximately 12 nm in diameter. PMID: 18302322

FAQs

Please fill out the Online Inquiry form located on the product page. Key product information has been pre-populated. You may also email your questions and inquiry requests to sales1@betalifesci.com. We will do our best to get back to you within 4 business hours.

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.