Recombinant Arabidopsis Thaliana Root Meristem Growth Factor 1 (RGF1) Protein (GST)

Name: Recombinant Arabidopsis Thaliana Root Meristem Growth Factor 1 (RGF1) Protein (GST)
Brand: Beta LifeScience
SKU: BLC-01625P
Availability: InStock

Greater than 85% as determined by SDS-PAGE.

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Product Overview

Description	Recombinant Arabidopsis Thaliana Root Meristem Growth Factor 1 (RGF1) Protein (GST) is produced by our E.coli expression system. This is a full length protein.
Purity	Greater than 85% as determined by SDS-PAGE.
Uniprotkb	Q3E880
Target Symbol	RGF1
Synonyms	AtRGF1
Species	Arabidopsis thaliana (Mouse-ear cress)
Expression System	E.coli
Tag	N-GST
Target Protein Sequence	DYSNPGHHPPRHN
Expression Range	104-116aa
Protein Length	Full Length of Mature Protein
Mol. Weight	28.2 kDa
Research Area	Signal Transduction
Form	Liquid or Lyophilized powder
Buffer	Liquid form: default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Lyophilized powder form: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Briefly centrifuged the vial prior to opening to bring the contents to the bottom. Reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. It is recommended to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. The default final concentration of glycerol is 50%.
Storage	1. Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. 2. Avoid repeated freeze-thaw cycles. 3. Store working aliquots at 4°C for up to one week. 4. In general, protein in liquid form is stable for up to 6 months at -20°C/-80°C. Protein in lyophilized powder form is stable for up to 12 months at -20°C/-80°C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Target Details

Target Function	Acts as a peptide hormone recognized by receptors (e.g. RGI1 and RGI2) to trigger signaling events including the regulation of RITF1 expression and leading to the production of reactive oxygen species (ROS) in roots to modulate meristem size. Signaling peptide (root growth factor) that maintains the postembryonic root stem cell niche in a PIN2-traffic dependent manner by regulating the expression levels and patterns of the transcription factor PLETHORA (PLT), mainly at the post-transcriptional level. Maintains the postembryonic root stem cell niche by regulating the expression levels and patterns of the transcription factor PLETHORA (e.g. PLT1 and PLT2), mainly at the post-transcriptional level. Influences circumferential cell number in the root meristem in response to Pi-deprivation. Regulates the pattern of root growth and lateral root development by modulating the length and the number of cortical cells in the root apical meristem (RAM), and the anticlinal asymmetric cell divisions in lateral root initiation cells.
Subcellular Location	[GLV11p]: Secreted.
Protein Families	RGF family
Database References	KEGG: ath:AT5G60810 STRING: 3702.AT5G60810.1 UniGene: PMID: 27229312 RGF2 influenced the longitudinal growth rate in the primary root in response to inorganic phosphate deprivation, whereas RGF1 affected circumferential cell number in the root meristem. PMID: 25856240 root meristem growth factor 1(At5g60810 peptide) required for maintenance of root stem cell niche and transit amplifying cell proliferation; it defines expression levels and patterns of stem cell transcription factor PLETHORA PMID: 20798316

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.