Rabbit Anti-Catalase Polyclonal Antibody

Beta LifeScience SKU/CAT #: BAR-0009A

Rabbit Anti-Catalase Polyclonal Antibody

Beta LifeScience SKU/CAT #: BAR-0009A
Regular price $525.00
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Size

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Product Overview

Description Rabbit Anti-Catalase Polyclonal Antibody is a highly specific immunodetection reagent designed for comprehensive recognition of human catalase, a key antioxidant enzyme responsible for the decomposition of hydrogen peroxide into water and oxygen. This antibody exhibits strong affinity for multiple epitopes across the tetrameric structure of catalase, enabling sensitive detection in techniques including western blotting, immunohistochemistry, and immunofluorescence. The polyclonal nature ensures reliable recognition of catalase's functional domains, making it particularly valuable for investigating oxidative stress responses, monitoring catalase expression in aging and age-related diseases, studying metabolic disorders where hydrogen peroxide homeostasis is disrupted, and evaluating the role of this critical antioxidant defense in cardiovascular, neurological, and pulmonary pathologies.
Host Species Rabbit
Form Liquid
Buffer Phosphate-buffered saline with 0.05% NaN3 as a preservative
Protein Concentration 1.96 mg/mL
Working Dilution > 1:2000 by indirect ELISA
Western Blot At 1:16000 dilution, antisera reacts to 0.75, 0.15, and 0.3 μg catalase and to 1 μL of red blood cell lysate using ALPH conjugated secondary antibody
Iep Single arc upon immunoelectrophoresis of red blood cell lysate.
Iso Type IgG
Storage/Handling 2 - 8 °C

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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