Rabbit Anti-Apolipoprotein Ai Polyclonal Antibody

Name: Rabbit Anti-Apolipoprotein Ai Polyclonal Antibody
Brand: Beta LifeScience
SKU: BAR-0012A
Price: 315.0 USD
Availability: InStock

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Price

Size

1 mL

Quantity

Submit an inquiry or email sales for a custom bulk quote. Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Description	Rabbit Anti-Apolipoprotein A-I Polyclonal Antibody is a highly specific immunodetection reagent designed for comprehensive recognition of human apolipoprotein A-I (apoA-I), the primary structural and functional protein component of high-density lipoprotein (HDL) particles. This antibody exhibits strong affinity for multiple epitopes across both lipid-free and lipid-associated forms of apoA-I, enabling sensitive detection in techniques including western blotting, ELISA, immunohistochemistry, and immunofluorescence. The polyclonal nature ensures reliable recognition of apoA-I's dynamic conformational states, making it particularly valuable for investigating reverse cholesterol transport, evaluating HDL metabolism and functionality, studying cardiovascular disease biomarkers, assessing the impact of apoA-I mutations on HDL deficiency states, and monitoring the therapeutic effects of apoA-I-based interventions in atherosclerosis research.
Host Species	Rabbit
Form	Liquid
Buffer	Phosphate-buffered saline with 0.05% NaN3 as a preservative
Protein Concentration	1.45 mg/mL
Working Dilution	> 1:1600 by indirect ELISA
Western Blot	At a 1:1000 dilution, reacts to 0.3 μg purified Apo AI antigen. Does not cross-react with 0.3 μg of Apo AII, B, CI, CII, CIII, or E.
Iep	Single arc vs purified antigen and whole human plasma.
Iso Type	IgG
Storage/Handling	2 - 8 °C

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.