Phospho-CDK2 (Y15) Recombinant Monoclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-06490A

Western BlotPositive WB detected in:Hela whole cell lysate, 293 whole cell lysate(treated with Pervanadate or not)All lanes:Phospho-CDK2 antibody at 0.8μg/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 34 KDaObserved band size: 34 KDa

Immunocytochemistry analysis of Phospho-CDK2 (Y15) Recombinant Monoclonal Antibody diluted at 1:80 and staining in Hela cells(treated with Pervanadate) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating Phospho-CDK2 in Hela whole cell lysate treated with PervanadateLane 1: Rabbit control IgG(1μg)instead of Phospho-CDK2 (Y15) Recombinant Monoclonal Antibody in Hela whole cell lysate treated with Pervanadate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: Phospho-CDK2 (Y15) Recombinant Monoclonal Antibody(3μg)+ Hela whole cell lysate treated with Pervanadate(1mg)Lane 3: Hela whole cell lysate treated with Pervanadate(20μg)
Phospho-CDK2 (Y15) Recombinant Monoclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-06490A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Product Title | Phospho-CDK2 (Y15) Recombinant Monoclonal Antibody |
Description | The recombinant antibody against CDK2 was produced using a synthesized peptide derived from Human Phospho-CDK2 (Y15) as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, IP. |
Uniprot Id - Alink | P24941 |
Reactivity | Human |
Target Name | CDK2 |
Target Synonyms | Cdc2 related protein kinase antibody; cdc2-related protein kinase antibody; CDC28 antibody; CDC2A antibody; Cdk 2 antibody; CDK1 antibody; CDK2 antibody; CDK2_HUMAN antibody; CDKN2 antibody; Cell devision kinase 2 antibody; Cell division protein kinase 2 antibody; Cyclin dependent kinase 2 antibody; cyclin dependent kinase 2-alpha antibody; Cyclin-dependent kinase 2 antibody; kinase Cdc2 antibody; MPF antibody; p33 protein kinase antibody; p33(CDK2) antibody |
Immunogen Description | A synthesized peptide derived from Human Phospho-CDK2 (Y15) |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Monoclonal |
Isotype | Rabbit IgG |
Purification Method | Affinity-chromatography purified |
Buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Form | Liquid |
Application | ELISA, WB, IHC, IP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |