MAPK1/MAPK3 Recombinant Monoclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-06560A

Western Blot | Positive WB detected in: THP-1 whole cell lysate, HEPG2 whole cell lysate,MCF7 whole cell lysate,293 whole cell lysate,K562 whole cell lysate,PC-3 whole cell lysate, Mouse brain tissue lysate | All lanes: ERK1/2 antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 42,44 kDa | Observed band size: 42,44 kDa

IHC image of MAPK1/MAPK3 Recombinant Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.64% DAB.

Overlay Peak curve showing Hela cells surface stained with MAPK1/MAPK3 Recombinant Monoclonal Antibody (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
MAPK1/MAPK3 Recombinant Monoclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-06560A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Product Title | MAPK1/MAPK3 Recombinant Monoclonal Antibody |
Description | The recombinant antibody against MAPK1/MAPK3 was produced using a synthesized peptide derived from Human MAPK1/MAPK3 as the immunogen. This antibody exists as a non-conjugated isotype Rabbit IgG, Affinity-chromatography purified. This antibody has been validated on ELISA, WB, IHC, FC. |
Uniprot Id - Alink | P27361 P28482 |
Reactivity | Human, Mouse |
Target Name | MAPK1/MAPK3 |
Target Synonyms | Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1), MAPK3, ERK1 PRKM3; Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2), MAPK1, ERK2 PRKM1 PRKM2 |
Immunogen Description | A synthesized peptide derived from Human MAPK1/MAPK3 |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Monoclonal |
Isotype | Rabbit IgG |
Purification Method | Affinity-chromatography purified |
Buffer | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Form | Liquid |
Application | ELISA, WB, IHC, FC |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |