Rabbit anti-Human SPICE1 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-02052A
Western blot | All lanes: SPICE1 antibody at 5.43 μg/ml | Lane 1: Mouse gonadal tissue | Lane 2: 293T whole cell lysate | Secondary | Goat polyclonal to rabbit IgG at 1/10000 dilution | Predicted band size: 96 kDa | Observed band size: 96, 45 kDa
Western blot | All lanes: SPICE1 antibody at 5.43 μg/ml | Lane 1: Mouse gonadal tissue | Lane 2: 293T whole cell lysate | Secondary | Goat polyclonal to rabbit IgG at 1/10000 dilution | Predicted band size: 96 kDa | Observed band size: 96, 45 kDa
Immunohistochemistry of paraffin-embedded human testis tissue using SPICE1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human testis tissue using SPICE1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using SPICE1 Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human thyroid tissue using SPICE1 Antibody at dilution of 1:100

Rabbit anti-Human SPICE1 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-02052A
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Product Overview

Product Title Rabbit anti-Human SPICE1 Polyclonal Antibody
Description The antibody against SPICE1 was raised in rabbit using the Recombinant Human Spindle and centriole-associated protein 1 protein (1-220AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC.
Uniprot Id - Alink Q8N0Z3
Host Species Rabbit
Reactivity Human, Mouse
Target Name SPICE1
Target Synonyms Coiled coil domain containing 52 antibody; Coiled coil domain containing protein 52 antibody; Coiled-coil domain-containing protein 52 antibody; FLJ26064 antibody; FLJ44949 antibody; SPICE antibody; SPICE_HUMAN antibody; spice1 antibody; Spindle and centriole protein antibody; Spindle and centriole-associated protein 1 antibody; Spindle and centriole-associated protein antibody
Immunogen Description Recombinant Human Spindle and centriole-associated protein 1 protein (1-220AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Antigen affinity purified
Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form Liquid
Application ELISA, WB, IHC
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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