Rabbit anti-Human SMPD2 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00548A

IHC image of the antibody diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of SMPD2 Antibody diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with SMPD2 Antibody at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Rabbit anti-Human SMPD2 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00548A
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Product Overview
Product Title | Rabbit anti-Human SMPD2 Polyclonal Antibody |
Description | The antibody against SMPD2 was raised in rabbit using the Recombinant Human Sphingomyelin phosphodiesterase 2 protein (199-301AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, IHC, IF. |
Uniprot Id - Alink | O60906 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | SMPD2 |
Target Synonyms | SMPD2; Sphingomyelin phosphodiesterase 2; Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase; nSMase1 |
Immunogen Description | Recombinant Human Sphingomyelin phosphodiesterase 2 protein (199-301AA) |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | >95%, Protein G purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, IHC, IF |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |