Rabbit anti-Human ROCK1 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00063A

Western Blot | Positive WB detected in: HL60 whole cell lysate | All lanes: ROCK1 antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 159 kDa | Observed band size: 159 kDa

IHC image of ROCK1 Antibody diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with ROCK1 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Rabbit anti-Human ROCK1 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00063A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.
Product Overview
Product Title | Rabbit anti-Human ROCK1 Polyclonal Antibody |
Description | The antibody against ROCK1 was raised in rabbit using the Recombinant Human Rho-associated protein kinase 1 protein (405-668AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF. |
Uniprot Id - Alink | Q13464 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | ROCK1 |
Target Synonyms | coiled-coil-containing protein kinase 1 antibody; coiled-coil-containing protein kinase I antibody; MGC131603 antibody; MGC43611 antibody; p160 Rhoassociated coiled coil-forming protein kinase antibody; p160 ROCK-1 antibody; p160 ROCK1 antibody; p160ROCK antibody; PRO0435 antibody; Renal carcinoma antigen NY REN 35 antibody; Renal carcinoma antigen NY-REN-35 antibody; Rho associated coiled coil containing protein kinase 1 antibody; Rho associated protein kinase 1 antibody; Rho kinase antibody; Rho-alpha kinase antibody; Rho-associated antibody; Rho-associated protein kinase 1 antibody; ROCK I antibody; ROCK-I antibody; ROCK1 antibody; ROCK1_HUMAN antibody; Rok antibody; rokalpha antibody |
Immunogen Description | Recombinant Human Rho-associated protein kinase 1 protein (405-668AA) |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | >95%, Protein G purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, IHC, IF |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |