Rabbit anti-Human PSMD14 Polyclonal Antibody

Name: Rabbit anti-Human PSMD14 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-00081A
Price: 299.0 USD
Availability: InStock

Western Blot | Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate, Jurkat whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, A549 whole cell lysate, 293T whole cell lysate, Rat liver tissue | All lanes: PSMD14 antibody at 1:2000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 35 kDa | Observed band size: 35 kDa

IHC image of PSMD14 Antibody diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of PSMD14 Antibody diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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Product Overview

Product Title	Rabbit anti-Human PSMD14 Polyclonal Antibody
Description	The antibody against PSMD14 was raised in rabbit using the Peptide sequence from Human 26S proteasome non-ATPase regulatory subunit 14 protein (264-283AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	O00487
Host Species	Rabbit
Reactivity	Human, Rat
Target Name	PSMD14
Target Synonyms	26S proteasome non-ATPase regulatory subunit 14 antibody; 26S proteasome regulatory subunit rpn11 antibody; 26S proteasome-associated PAD1 homolog 1 antibody; 26S proteasome-associated PAD1 homolog antibody; PAD1 antibody; PAD1, yeast, homolog of antibody; POH1 antibody; Proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 antibody; Proteasome 26S subunit non ATPase 14 antibody; PSDE_HUMAN antibody; Psmd14 antibody; RPN11 antibody; Testis tissue sperm binding protein Li 69n antibody
Immunogen Description	Peptide sequence from Human 26S proteasome non-ATPase regulatory subunit 14 protein (264-283AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Antigen affinity purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.