Mouse anti-Human PRDX3 Monoclonal antibody

Beta LifeScience SKU/CAT #: BLC-06396A
Western Blot Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate All lanes: PRDX3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 KDa Exposure time：5min
Western Blot Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate All lanes: PRDX3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 KDa Exposure time：5min
IHC image of PRDX3 Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PRDX3 Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PRDX3 Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human endometrial cance tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PRDX3 Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human endometrial cance tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot Positive WB detected in: Hela whole cell lysate, MCF7 whole cell lysate, 293T whole cell lysate, JK whole cell lysate, HepG2 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate All lanes: PRDX3 antibody at 1:1000 Secondary Goat polyclonal to mouse IgG at 1/50000 dilution Predicted band size: 28 kDa Observed band size: 28 KDa Exposure time：5min
IHC image of PRDX3 Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of PRDX3 Monoclonal Antibody diluted at 1:50 and staining in paraffin-embedded human endometrial cance tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Mouse anti-Human PRDX3 Monoclonal antibody

Beta LifeScience SKU/CAT #: BLC-06396A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title Mouse anti-Human PRDX3 Monoclonal antibody
Description The antibody against PRDX3 was raised in mouse using the Recombinant Human Thioredoxin-dependent peroxide reductase, mitochondrial protein (63-256AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG2b, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF, FC.
Uniprot Id - Alink P30048
Host Species Mouse
Reactivity Human
Target Name PRDX3
Target Synonyms Antioxidant protein 1 antibody; AOP 1 antibody; AOP-1 antibody; AOP1 antibody; HBC189 antibody; MER5 antibody; MGC104387 antibody; MGC24293 antibody; mitochondrial antibody; peroxiredoxin 3 antibody; Peroxiredoxin III antibody; Peroxiredoxin-3 antibody; PRDX3 antibody; PRDX3_HUMAN antibody; PRO1748 antibody; Protein MER5 homolog antibody; PRX III antibody; Prx-III antibody; PRX3 antibody; SP 22 antibody; SP-22 antibody; SP22 antibody; Thioredoxin dependent peroxide reductase mitochondrial antibody; Thioredoxin-dependent peroxide reductase antibody
Immunogen Description Recombinant Human Thioredoxin-dependent peroxide reductase, mitochondrial protein (63-256AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Monoclonal
Isotype IgG2b
Purification Method >95%, Protein G purified
Buffer 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Application ELISA, WB, IHC, IF, FC
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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