Rabbit anti-Human PHB Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-06216A
IHC image of the antibody diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of the antibody diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot | Positive WB detected in: Raji whole cell lysate | All lanes: PHB antibody at 4.5µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 30, 18 kDa | Observed band size: 30 kDa
Western Blot | Positive WB detected in: Raji whole cell lysate | All lanes: PHB antibody at 4.5µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 30, 18 kDa | Observed band size: 30 kDa
Immunoprecipitating PHB in HEK293 whole cell lysate | Lane 1: Rabbit control IgG (1µg) instead of PHB Antibody in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: PHB Antibody (8µg) + HEK293 whole cell lysate (500µg) | Lane 3: HEK293 whole cell lysate (10µg)
Immunoprecipitating PHB in HEK293 whole cell lysate | Lane 1: Rabbit control IgG (1µg) instead of PHB Antibody in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: PHB Antibody (8µg) + HEK293 whole cell lysate (500µg) | Lane 3: HEK293 whole cell lysate (10µg)
IHC image of the antibody diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western Blot | Positive WB detected in: Raji whole cell lysate | All lanes: PHB antibody at 4.5µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 30, 18 kDa | Observed band size: 30 kDa
Immunoprecipitating PHB in HEK293 whole cell lysate | Lane 1: Rabbit control IgG (1µg) instead of PHB Antibody in HEK293 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: PHB Antibody (8µg) + HEK293 whole cell lysate (500µg) | Lane 3: HEK293 whole cell lysate (10µg)

Rabbit anti-Human PHB Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-06216A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title Rabbit anti-Human PHB Polyclonal Antibody
Description The antibody against PHB was raised in rabbit using the Recombinant Human Prohibitin protein (1-272AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IP.
Uniprot Id - Alink P35232
Host Species Rabbit
Reactivity Human
Target Name PHB
Target Synonyms Epididymis luminal protein 215 antibody; Epididymis secretory sperm binding protein Li 54e antibody; HEL 215 antibody; HEL S 54e antibody; PHB antibody; PHB_HUMAN antibody; PHB1 antibody; Prohibitin antibody
Immunogen Description Recombinant Human Prohibitin protein (1-272AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Buffer 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Application ELISA, WB, IHC, IP
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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