Rabbit anti-Human NUP98 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-00709A
Western Blot | Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate | All lanes: NUP98 antibody at 3.2μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 198, 188, 98, 97, 196, 187 kDa | Observed band size: 105 kDa
Western Blot | Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate | All lanes: NUP98 antibody at 3.2μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 198, 188, 98, 97, 196, 187 kDa | Observed band size: 105 kDa
Immunofluorescence staining of A549 cells with NUP98 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunofluorescence staining of A549 cells with NUP98 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating NUP98 in Jurkat whole cell lysate | Lane 1: Rabbit control IgG instead of NUP98 Antibody in Jurkat whole cell lysate. | For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: NUP98 Antibody (8μg) + Jurkat whole cell lysate (500μg) | Lane 3: Jurkat whole cell lysate (20μg)
Immunoprecipitating NUP98 in Jurkat whole cell lysate | Lane 1: Rabbit control IgG instead of NUP98 Antibody in Jurkat whole cell lysate. | For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: NUP98 Antibody (8μg) + Jurkat whole cell lysate (500μg) | Lane 3: Jurkat whole cell lysate (20μg)
Western Blot | Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate | All lanes: NUP98 antibody at 3.2μg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 198, 188, 98, 97, 196, 187 kDa | Observed band size: 105 kDa
Immunofluorescence staining of A549 cells with NUP98 Antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunoprecipitating NUP98 in Jurkat whole cell lysate | Lane 1: Rabbit control IgG instead of NUP98 Antibody in Jurkat whole cell lysate. | For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: NUP98 Antibody (8μg) + Jurkat whole cell lysate (500μg) | Lane 3: Jurkat whole cell lysate (20μg)

Rabbit anti-Human NUP98 Polyclonal Antibody

Beta LifeScience SKU/CAT #: BLC-00709A
Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

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Product Overview

Product Title Rabbit anti-Human NUP98 Polyclonal Antibody
Description The antibody against NUP98 was raised in rabbit using the Recombinant Human Nuclear pore complex protein Nup98-Nup96 protein (965-1110AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IF, IP.
Uniprot Id - Alink P52948
Host Species Rabbit
Reactivity Human
Target Name NUP98
Target Synonyms 96 kDa nucleoporin antibody; 98 kDa nucleoporin antibody; ADAR2 antibody; ADIR2 antibody; GLFG-repeat containing nucleoporin antibody; Nuclear pore complex protein Nup96 antibody; Nuclear pore complex protein Nup98 Nup96 antibody; Nucleoporin 98kD antibody; nucleoporin 98kDa antibody; Nucleoporin Nup96 antibody; Nucleoporin Nup98 antibody; NUP196 antibody; NUP96 antibody; Nup98 antibody; Nup98-Nup96 antibody; NUP98_HUMAN antibody
Immunogen Description Recombinant Human Nuclear pore complex protein Nup98-Nup96 protein (965-1110AA)
Immunogen Species Human
Immunogen Sequence Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Buffer 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Application ELISA, WB, IF, IP
Storage Conditions Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.

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