Rabbit anti-Human MKLN1 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00212A

Western Blot | Positive WB detected in: Hela whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, Rat brain tissue | All lanes: MKLN1 antibody at 2.7µg/ml | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 85, 61 kDa | Observed band size: 85 kDa

IHC image of MKLN1 Antibody diluted at 1:400 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of HepG2 cells with MKLN1 Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Rabbit anti-Human MKLN1 Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00212A
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Product Overview
Product Title | Rabbit anti-Human MKLN1 Polyclonal Antibody |
Description | The antibody against MKLN1 was raised in rabbit using the Recombinant Human Muskelin protein (488-614AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, purified by protein G with a purity greater than 95%. This antibody has been validated on ELISA, WB, IHC, IF. |
Uniprot Id - Alink | Q9UL63 |
Host Species | Rabbit |
Reactivity | Human, Rat |
Target Name | MKLN1 |
Target Synonyms | MKLN 1 antibody; Mkln1 antibody; MKLN1_HUMAN antibody; muskelin 1, intracellular mediator containing kelch motifs antibody; Muskelin antibody; TWA2 antibody; Two-hybrid-associated protein with Ranbpm 2 antibody |
Immunogen Description | Recombinant Human Muskelin protein (488-614AA) |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | >95%, Protein G purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, IHC, IF |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |