Rabbit anti-Human MAP4K4 Polyclonal Antibody

Name: Rabbit anti-Human MAP4K4 Polyclonal Antibody
Brand: Beta LifeScience
SKU: BLC-00021A
Price: 299.0 USD
Availability: InStock

Western Blot | Positive WB detected in: Hela whole cell lysate, PC-3 whole cell lysate, Raji whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate, Mouse liver tissue | All lanes: MAP4K4 antibody at 1:1000 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 143, 139, 151, 134, 142, 146 kDa | Observed band size: 143 kDa

IHC image of MAP4K4 Antibody diluted at 1:100 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of MAP4K4 Antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Our products are highly customizable to meet your specific needs. You can choose options such as endotoxin removal, liquid or lyophilized forms, preferred tags, and the desired functional sequence range for proteins. Submitting a written inquiry expedites the quoting process.

Product Overview

Product Title	Rabbit anti-Human MAP4K4 Polyclonal Antibody
Description	The antibody against MAP4K4 was raised in rabbit using the Recombinant Human Mitogen-activated protein kinase kinase kinase kinase 4 protein (866-1104AA) as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, IHC, IF.
Uniprot Id - Alink	O95819
Host Species	Rabbit
Reactivity	Human, Mouse
Target Name	MAP4K4
Target Synonyms	FLH21957 antibody; FLJ10410 antibody; FLJ20373 antibody; FLJ90111 antibody; Hepatocyte progenitor kinase like/germinal center kinase like kinase antibody; HGK antibody; HPK/GCK like kinase antibody; HPK/GCK like kinase HGK antibody; HPK/GCK-like kinase HGK antibody; KIAA0687 antibody; M4K4_HUMAN antibody; MAP4K4 antibody; MAPK/ERK kinase kinase kinase 4 antibody; MEK kinase kinase 4 antibody; MEKKK 4 antibody; MEKKK4 antibody; Mitogen activated protein kinase kinase kinase kinase 4 antibody; Mitogen-activated protein kinase kinase kinase kinase 4 antibody; Nck interacting kinase antibody; Nck-interacting kinase antibody; NIK antibody
Immunogen Description	Recombinant Human Mitogen-activated protein kinase kinase kinase kinase 4 protein (866-1104AA)
Immunogen Species	Human
Immunogen Sequence	Complete sequences for the immunogen, target protein, and peptides are available upon request.
Conjugate	Non-conjugated
Clonality	Polyclonal
Isotype	IgG
Purification Method	Antigen affinity purified
Buffer	0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form	Liquid
Application	ELISA, WB, IHC, IF
Storage Conditions	Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.