Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein, Active

Name: Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein, Active
Brand: Beta LifeScience
SKU: BLK-01060P-100UG
Availability: InStock

Human ITGAM&ITGB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Collections: High-quality recombinant proteins

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Product Overview

Description	Recombinant Human Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700 (ITGB2) basic tail.
Purity	> 95% as determined by Tris-Bis PAGE
Accession	P11215-1(ITGAM)&P05107-1(ITGB2)
Target Symbol	Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer
Species	Human
Expression System	HEK293
Tag	C-His
Expression Range	Phe17-Asn1104 (ITGAM) acidic tail & Gln23-Asn700 (ITGB2) basic tail
Mol. Weight	The protein has a predicted MW of 126.10 kDa (ITGAM) and 79.50 kDa (ITGB2). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM) and 90-100 kDa (ITGB2) based on Tris-Bis PAGE result.
Form	Lyophilized
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Endotoxin	Less than 1EU per ug by the LAL method.
Activity	Immobilized Human ITGAM&ITGB2, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. Contact us for detailed testing images.
Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Shipping	Shipped at ambient temperature.
Gene Background	Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DCSIGN[2].

FAQs

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Proteins are sensitive to heat, and freeze-drying can preserve the activity of the majority of proteins. It improves protein stability, extends storage time, and reduces shipping costs. However, freeze-drying can also lead to the loss of the active portion of the protein and cause aggregation and denaturation issues. Nonetheless, these adverse effects can be minimized by incorporating protective agents such as stabilizers, additives, and excipients, and by carefully controlling various lyophilization conditions.

Commonly used protectant include saccharides, polyols, polymers, surfactants, some proteins and amino acids etc. We usually add 8% (mass ratio by volume) of trehalose and mannitol as lyoprotectant. Trehalose can significantly prevent the alter of the protein secondary structure, the extension and aggregation of proteins during freeze-drying process; mannitol is also a universal applied protectant and fillers, which can reduce the aggregation of certain proteins after lyophilization.

Our protein products do not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, etc., and when lyophilized with the solution with the lowest salt content, they often cannot form A white grid structure, but a small amount of protein is deposited in the tube during the freeze-drying process, forming a thin or invisible transparent protein layer.

Reminder: Before opening the tube cap, we recommend that you quickly centrifuge for 20-30 seconds in a small centrifuge, so that the protein attached to the tube cap or the tube wall can be aggregated at the bottom of the tube. Our quality control procedures ensure that each tube contains the correct amount of protein, and although sometimes you can't see the protein powder, the amount of protein in the tube is still very precise.

To learn more about how to properly dissolve the lyophilized recombinant protein, please visit Lyophilization FAQs.