Rabbit anti-Human HIST1H2BC Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00199A

Immunocytochemistry analysis of the antibody diluted at 1:5 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with Acetyl-HIST1H2BC (K108) Antibody at 1:2.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Chromatin Immunoprecipitation Hela (4*106, treated with 30mM sodium butyrate for 4h) were treated with Benzanase, sonicated, and immunoprecipitated with 5µg anti-HIST1H2BC (Acetyl-HIST1H2BC (K108) Antibody) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Rabbit anti-Human HIST1H2BC Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00199A
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Product Overview
Product Title | Rabbit anti-Human HIST1H2BC Polyclonal Antibody |
Description | The antibody against HIST1H2BC was raised in rabbit using the Peptide sequence around site of Acetyl-Lys (108) derived from Human Histone H2B type 1-C/E/F/G/I as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, ICC, IF, ChIP. |
Uniprot Id - Alink | P62807 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | HIST1H2BC |
Target Synonyms | H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody |
Immunogen Description | Peptide sequence around site of Acetyl-Lys (108) derived from Human Histone H2B type 1-C/E/F/G/I |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Antigen affinity purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, ICC, IF, ChIP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |