Rabbit anti-Human HIST1H2BC Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00169A

Western Blot | Detected samples: Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h | All lanes: HIST1H2BC antibody at 1:100 | Secondary | Goat polyclonal to rabbit IgG at 1/50000 dilution | Predicted band size: 14 kDa | Observed band size: 14 kDa

Immunocytochemistry analysis of Acetyl-HIST1H2BC (K24) Antibody diluted at 1:10 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunoprecipitating HIST1H2BC in A549 whole cell lysate | Lane 1: Rabbit control IgG instead of Acetyl-HIST1H2BC (K24) Antibody in A549 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) | Lane 2: Acetyl-HIST1H2BC (K24) Antibody (5µg) + A549 whole cell lysate (500µg) | Lane 3: A549 whole cell lysate (20µg)
Rabbit anti-Human HIST1H2BC Polyclonal Antibody
Beta LifeScience
SKU/CAT #: BLC-00169A
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Product Overview
Product Title | Rabbit anti-Human HIST1H2BC Polyclonal Antibody |
Description | The antibody against HIST1H2BC was raised in rabbit using the Peptide sequence around site of Acetyl-Lys (24) derived from Human Histone H2B type 1-C/E/F/G/I as the immunogen. This antibody exists as a non-conjugated isotype IgG, Antigen affinity purified. This antibody has been validated on ELISA, WB, ICC, IP. |
Uniprot Id - Alink | P62807 |
Host Species | Rabbit |
Reactivity | Human |
Target Name | HIST1H2BC |
Target Synonyms | H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody |
Immunogen Description | Peptide sequence around site of Acetyl-Lys (24) derived from Human Histone H2B type 1-C/E/F/G/I |
Immunogen Species | Human |
Immunogen Sequence | Complete sequences for the immunogen, target protein, and peptides are available upon request. |
Conjugate | Non-conjugated |
Clonality | Polyclonal |
Isotype | IgG |
Purification Method | Antigen affinity purified |
Buffer | 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
Form | Liquid |
Application | ELISA, WB, ICC, IP |
Storage Conditions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |